Protective effect of allopurinol on experimental ovarian ischemia-reperfusion injury model of rats
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Science Printers and Publishers Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
OBJECTIVE: To investigate the effect of allopurinol on an experimentally induced ovarian ischemia-reperfusion model. STUDY DESIGN: Female rats in the estrous cycle (n=32) were divided into sham, ischemia, ischemia-reperfusion, and ischemia-reperfusion+allopurinol-treated groups. In the sham group the ovaries were opened and closed. In the ischemia group the ovaries were sealed for 2-hour ischemia. In the ischemia-reperfusion group, after ischemia, 2.5 hours of reperfusion was done. In the ischemia-reperfusion+allopurinol group, 3 hours after ischemia-reperfusion, 50 mg/kg allopurinol was administered. RESULTS: In the allopurinol-administered group, MDA levels were decreased. GSH values were decreased in the ischemia and ischemia-reperfusion group but increased in the allopurinol-treated group as compared to the control group. Caspase-3 expression was positive in enlarged corpus luteum cells. sFlt-1 expression was positive in vascular endothelial cells between preantral and antral follicles and some macrophages but negative in granular cells. In the ischemia group, sFlt-1 expression was positive in degenerative preantral and antral follicle cells, endothelial cells, and intense inflammatory cells. In the ischemia-reperfusion group, increased sFlt-1 expression was observed in luteal cells of the corpus luteum, vascular endothelial, and inflammatory cells. In the ischemia-reperfusion+allopurinol group, granular cells and corpus luteum cells showed decreased sFlt-1 expression, while being positive in vascular endothelial cells. CONCLUSION: Allopurinol inhibits development of apoptosis and reduces oxidative load in the ischemia-reperfusion stage, thus protecting the ovary from damage. © Science Printers and Publishers, Inc.
Açıklama
Anahtar Kelimeler
Allopurinol, Caspase-3, FLT1 protein, Fms-like tyrosine kinase-1, Ischemia, Ischemia-reperfusion injury, Ovarian diseases, Ovarian ischemia, Ovarian torsion, Ovary, Oxidative stress, Rats, Reperfusion injury, SFlt-1, Vascular endothelial growth factor receptor-1
Kaynak
Analytical and Quantitative Cytopathology and Histopathology
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
42
Sayı
1
