Enzyme immobilization onto carbon fiber electrodes by electrochemicalpolymerization
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
New enzyme electrodes were fabricated by using carbon fiber as electrode substrate. Electrodes were obtained by immobilization of invertase in the conductive polypyrrole matrix during electrochemical polymerization onto laboratory–made carbon fiber electrodes. Maximum reaction rate, ($V_{max}$) and substrate affinity, ($K_m$), of immobilized enzyme were determined as 56.7 ?mol $min^{-1}$ $electrode^{-1}$and 1.57 mM respectively. The effect of conditions on enzyme activity was investigated. It was found that the optimum temperature, optimum pH value, linear working range are 30 ºC, pH 5.0 and 0.0025?0.0200 M respectively. The electrodes were examined for operational stability. The results were compared with the previous studies in which invertase was immobilized onto platinum substrate.
Açıklama
Anahtar Kelimeler
Kaynak
Karaelmas Fen ve Mühendislik Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
10
Sayı
1
