Electrochemical Phenol Biosensor Configurations Based on Nanobiocomposites
Küçük Resim Yok
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Myu, Scientific Publishing Division
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Different biosensor configurations were constructed based on nanobiocomposites for the detection of phenol. The immobilization of laccase (TvLac) was achieved on a glassy carbon (GC) with polypyrrole (PPy), polypyrrole-multiwall carbon nanotube (PPy-MWCNT), and polypyrrole-multiwall carbon nanotube-Prussian blue (PPy-MWCNT-PB) composites via electrochemical polymerization. A comparative study was made of the analytical properties of the biosensors corresponding to the three configurations, namely, GC/PPy-TvLac, GC/PPy-TvLac-MWCNT, and GC/PPy-TvLac-MWCNT-PB. All the configurations indicated that the (TvLac-MWCNT-PB) nanobiocomposites were entrapped within the porous PPy film and resulted in a hybrid film that showed a high electrocatalytic ability toward the oxidation of phenol at a potential of -200 mV vs Ag/AgCl. The GC/PPy-TvLac-MWCNT-PB working electrode gave performance characteristics with high sensitivity (309.1 nA/mu M), low detection limit, and good stability. This electrode allowed the determination of phenol in the 0.2 -2.56 mu M concentration range. The sensitivities (S/N = 3) for phenol obtained from the different working electrodes were found to be 4.56, 91.03, and 309.1 mu M, respectively.
Açıklama
Anahtar Kelimeler
carbon nanotubes, electropolymerization, laccase, phenol, Prussian blue
Kaynak
Sensors and Materials
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
24
Sayı
3