Electrochemical Phenol Biosensor Configurations Based on Nanobiocomposites

Küçük Resim Yok

Tarih

2012

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Myu, Scientific Publishing Division

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Different biosensor configurations were constructed based on nanobiocomposites for the detection of phenol. The immobilization of laccase (TvLac) was achieved on a glassy carbon (GC) with polypyrrole (PPy), polypyrrole-multiwall carbon nanotube (PPy-MWCNT), and polypyrrole-multiwall carbon nanotube-Prussian blue (PPy-MWCNT-PB) composites via electrochemical polymerization. A comparative study was made of the analytical properties of the biosensors corresponding to the three configurations, namely, GC/PPy-TvLac, GC/PPy-TvLac-MWCNT, and GC/PPy-TvLac-MWCNT-PB. All the configurations indicated that the (TvLac-MWCNT-PB) nanobiocomposites were entrapped within the porous PPy film and resulted in a hybrid film that showed a high electrocatalytic ability toward the oxidation of phenol at a potential of -200 mV vs Ag/AgCl. The GC/PPy-TvLac-MWCNT-PB working electrode gave performance characteristics with high sensitivity (309.1 nA/mu M), low detection limit, and good stability. This electrode allowed the determination of phenol in the 0.2 -2.56 mu M concentration range. The sensitivities (S/N = 3) for phenol obtained from the different working electrodes were found to be 4.56, 91.03, and 309.1 mu M, respectively.

Açıklama

Anahtar Kelimeler

carbon nanotubes, electropolymerization, laccase, phenol, Prussian blue

Kaynak

Sensors and Materials

WoS Q Değeri

Q4

Scopus Q Değeri

Cilt

24

Sayı

3

Künye