Aggul, Ahmet GokhanUzun, NaimKuzu, MuslumTaslimi, ParhamGulcin, Ilhami2024-09-292024-09-2920220365-62331521-4184https://doi.org/10.1002/ardp.202100476https://hdl.handle.net/20.500.14619/3643This paper presents experimental and molecular docking studies on the inhibitory effects of tyrosol, hydroxytyrosol, luteolin, diosmetin, caffeic acid, luteolin 7-O-glycoside, and apigenin 7-O-glycoside from olive (Olea europaea L.) leaf against human carbonic anhydrase (hCA, E.C.4.2.1.1) isozymes I and II. After these isozymes were separately purified, their activities were determined using the esterase activity. IC50 values for hCA I and II were calculated as 2.02-11.38 mu M and 2.23-9.05 mu M, respectively. The compounds were identified as CA inhibitors, with K-i values in the ranges of 1.66-9.17 mu M for the hCA I isozyme and 1.49-14.21 mu M for hCA II. The inhibitory effects of these natural compounds were also compared to acetazolamide, which is a potent inhibitor of both CA isozymes. Our results may contribute to the synthesis of new CA inhibitors and pave the way for new drug design in the treatment of a number of diseases including cancer, obesity, diabetes, and glaucoma.eninfo:eu-repo/semantics/closedAccesscarbonic anhydraseenzyme inhibitionmolecular dockingolive leafphenolic compoundArticle10.1002/ardp.2021004762-s2.0-85126563829635306678Q2355WOS:000770784600001Q1