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Öğe Biochemical investigation of the toxic effects of acrylamide administration during pregnancy on the liver of mother and fetus and the protective role of vitamin E(Taylor & Francis Ltd, 2017) Erdemli, Mehmet Erman; Altinoz, Eyup; Aksungur, Zeynep; Turkoz, Yusuf; Dogan, Zumrut; Bag, Harika GozukaraObjectives: To investigate the toxic effects occurring in the liver tissues of the pregnant rats and the fetuses, which are administered acrylamide and vitamin E as a protector during pregnancy. Materials and methods: This research was conducted with the permission of Laboratory Animals Ethical Board of Inonu University Faculty of Medicine. Forty rats, of which their pregnancy is validated via vaginal smear, were distributed into five different groups. On the 20th day of pregnancy, pregnant rats and fetuses are decapitated. Malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant status (TAS), total oxidant status (TOS) and xanthine oxidase (XO) levels were measured in the liver samples taken from mother and fetuses. Results: It was detected that acrylamide administered during pregnancy increased MDA, TOS, XO levels statistically significantly and decreased the GSH level (p <= 0.05) in the pregnant rat liver tissue when compared to all other groups. In the vitamin E administered group; GSH, TAS levels significantly increased statistically and TOS and XO levels dropped to levels of the control group (p <= 0.05), in comparison to all other groups. Among all groups, no biochemical changes were observed in the fetus liver tissue (p > 0.05). Conclusion: The liver of pregnant rats functions as a protective pre-filter by detoxifying acrylamide effectively and the acrylamide that reaches fetus liver is detoxified by the cytochrome P-450 system of the fetus liver. To be able to figure out the biochemical mechanism, more advanced studies are needed.Öğe Can crocin play a preventive role in Wistar rats with carbon tetrachloride-induced nephrotoxicity?(Mashhad Univ Med Sciences, 2018) Erdemli, Mehmet Erman; Gul, Mehmet; Altinoz, Eyup; Aksungur, Zeynep; Gul, Semir; Bag, Harika GozukaraObjective(s): To investigate protective role of crocin by attempting to create nephrotoxicity with carbon tetrachloride. Materials and Methods: Ethics committee approval was obtained and 50 male Wistar rats were randomly divided into 5 groups that included 10 rats each: Control, Corn oil, Crocin, Carbon tetrachloride (CCl4), and Crocin + Carbon tetrachloride. Following the experiments, the rats were decapitated under anesthesia and incised kidney tissues were subjected to biochemical and histological examinations. Results: In the CCl4 administered group, MDA, TOS, Bun, and creatinine levels increased, GSH, SOD, CAT, and TAS levels decreased (P <= 0.05), glomerular collapse in kidney sections, narrowing and local occlusion in Bowman's space in certain glomeruli, inflammatory cell infiltration and congestion were observed when compared to all other groups. There was a significant decrease in increased MDA, TOS, Bun, and creatinine levels, and a significant increase in decreased GSH, SOD, CAT, and TAS levels in CCl4 + crocin administered group compared to the CCl4 group (P <= 0.05), local minimal glomerular damage, tubular damage, inflammatory infiltration, and vascular collagen symptoms were observed in kidney sections, however significant improvement was observed in damage findings when compared to the CCl4 group. Conclusion: At this dose and time interval, against a highly toxic chemical such as CCl4, crocin was able to suppress oxidative stress by playing a protective role in the kidney tissue.Öğe Crocin protects intestine tissue against carbon tetrachloride-mediated oxidative stress in rats(Aepress Sro, 2018) Cosgun, Binnaz E.; Erdemli, Mehmet E.; Gul, Mehmet; Gul, Semir; Bag, Harika G.; Aksungur, Zeynep; Altinoz, EyupSaffron is used in traditional medicine for its hypolipidemic, anti-inflammatory and anti carcinogenic properties as a natural remedy in treatment of diseases. The objective of the present study was to demonstrate the protective effect of crocin (one of the main ingredients of saffron) on carbon tetrachloride (CCl4) damage in intestinal mucosa. MDA, GSH, SOD, CAT, TAS and TOS levels were measured in experimental animal tissue samples and these were compared with histologic lesions induced by CCl4.CCl4 caused an increase in MDA, SOD, CAT and TOS levels and a significant decrease in GSH and TAS levels in rat intestinal tracts. After crocin treatment, serious improvements were observed in histological lesions and biochemical results in the intestinal tract. In conclusion, crocin inhibited the toxic effects induced by CCl4 in the intestine by its strong antioxidant properties.Öğe The effects of acrylamide and vitamin E on kidneys in pregnancy: an experimental study(Taylor & Francis Ltd, 2019) Erdemli, Mehmet Erman; Aksungur, Zeynep; Gul, Mehmet; Yigitcan, Birgul; Bag, Harika Gozukara; Altinoz, Eyup; Turkoz, YusufObjectives: The objective of this study is to investigate possible damages to kidney tissues of pregnant rats and their fetuses exposed to acrylamide during pregnancy and possible protective effects of vitamin E against these damages. Material and methods: Rats were randomly assigned to five groups of control, corn oil, vitamin E, acrylamide, vitamin E + acrylamide, six pregnant rats in each. Mother and fetal kidney tissues were examined for malondialdehyde (MDA), reductase glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), total antioxidant status (TAS), total oxidant status (TOS), urea, creatine, trace elements such as Zn and Cu in the serum and histopathological analyses were conducted. Results: It was determined that acrylamide, administered during pregnancy, statistically significantly increased MDA and TOS levels, maternal serum urea, creatinine, and Zn levels, while it decreased GSH, TAS, SOD, and CAT levels (p <= .05) when compared with all other groups in the kidney tissues of pregnant rats and their fetuses and caused tubular degeneration, hemorrhage, narrowing, and closure in Bowman's space, and, in the E vitamin group, it statistically significantly increased GSH, TAS, SOD, CAT, urea, creatinine, and Zn levels when compared with other groups and lowered TOS and MDA levels to those of the control group (p < .05) and there were no differences between the groups histologically. Conclusion: It was observed that acrylamide administered during pregnancy caused oxidative stress in kidney tissues of mother rats and their fetuses, resulting in tissue damage, and vitamin E application, which is considered to be a powerful antioxidant, inhibited oxidative stress.Öğe Gebe ratlara uygulanan akrilamid ve e vitaminin plasenta dokusu üzerine olası etkilerinin araştırılması(2017) Erdemlı, Mehmet Erman; Altınöz, Eyüp; Aksungur, Zeynep; Doğan, Zümrüt; Gözükara Bağ, Harika Gözde; Türköz, YusufGebelik boyunca akrilamid (AA) ve koruyucu olarak E vitamini (E vit) uygulanan ratların plasenta dokularında meydana gelen değişimlerin incelenmesi amaçlanmıştır. Vajinal smear ile gebelikleri doğrulanan 30 rat; Kontrol, Mısır yağı, E vitamini, Akrilamid, E vitamini + Akrilamid: grupları olmak üzere rastgele seçilerek 5 farklı gruba ayrıldı. Gebeliğin 20. gününde gebe ratlar dekapite edildi. Plasenta dokularında; malondialdehid (MDA), redükte glutatyon (GSH), total antioksidan kapasite (TAS), total oksidan kapasite (TOS) ve ksantin oksidaz (XO) düzeyleri ölçüldü. Gebe ratların plasenta dokusunda: gebelik boyunca uygulanan akrilamidin; diğer tüm gruplarla kıyaslandığında MDA, TOS, XO seviyelerini istatistiki olarak anlamlı ölçüde arttırdığı, GSH ve TAS seviyesini ise azalttığı, E vitamini uygulanan grupta ise, diğer tüm gruplara kıyasla GSH, TAS seviyelerini istatistiki olarak anlamlı ölçüde arttığını ve TOS ile XO seviyelerini ise kontrol grubu seviyelerine düşürdüğü tespit edildi. Ağız yoluyla gebe ratlara verilen AA, plasenta dokusunda MDA, XO ve TOS seviyelerini arttırarak antioksidan/oksidan dengeyi oksidanlar lehine değiştirdiği ve oksidatif strese neden olduğu, E vitamini uygulamasının ise antioksidan/oksidan dengeyi normal sınırlara getirerek oksidatif stres kaynaklı toksikasyonları önlediği görülmektedir.Öğe Hepatoprotective effects of crocin on biochemical and histopathological alterations following acrylamide-induced liver injury in Wistar rats(Elsevier France-Editions Scientifiques Medicales Elsevier, 2017) Gedik, Sema; Erdemli, Mehmet Erman; Gul, Mehmet; Yigitcan, Birgul; Bag, Harika Gozukara; Aksungur, Zeynep; Altinoz, EyupThe objective of the present study is the treatment of oxidative damage caused by acrylamide induced oxidative stress in rats with the administration of a strong antioxidant, namely crocin. High acrylamide (AA) levels have genotoxic, carcinogenic and neurotoxic effects on living organisms. In the present study, 40 Wistar rats were randomly divided into four equal groups. These groups were control, acrylamide (25 mg/kg), crocin (50 mg/kg), acrylamide + crocin (25 mg/kg acrylamide and 50 mg/kg crocin) groups. At the end of the application, biochemical and histological variations were examined in liver and blood samples. It was observed that acrylamide administration significantly decreased liver GSH and TAS levels when compared to the control group. On the contrary, it was also observed that AST, ALT, ALP, SOD and CAT activities and TOS and MDA levels increased as a result of acrylamide administration. Histopathological examinations demonstrated inflammatory cell infiltration, hepatocellular necrosis and hemorrhage areas in AA group liver sections. Furthermore, intracytoplasmic vacuolization was detected in hepatocytes. After crocin treatment, it was observed that GSH and TAS levels increased while AST, ALT, ALP, SOD and CAT activities and TOS and MDA levels decreased. Significant decreases were observed in inflammatory cell infiltration and vascular congestion in liver sections and intracytoplasmic vacuolization in hepatocytes after the crocin treatment, while no hepatocellular necrosis and hemorrhages were observed. In the present study, it was demonstrated that crocin treatment removed acrylamide induced liver damage due to the strong antioxidant properties of crocin.Öğe Investigation of wet cupping therapy's effect on oxidative stress based on biochemical parameters(Elsevier Science Inc, 2019) Ersoy, Suleyman; Altinoz, Eyup; Benli, Ali Ramazan; Erdemli, Mehmet Erman; Aksungur, Zeynep; Bag, Harika Gozukara; Engin, Velittin SelcukIntroduction: Wet cupping therapy is one of the main applications which has been used in the Unani medicine system. Current literature explaining how this traditional treatment method works is still limited. The aim of this study was to investigate whether wet cupping therapy could affect reactive oxygen species and antioxidant levels. Methods: This was a single arm pre and posttest intervention study conducted in Karabuk University Teaching and Research Hospital (KUTRH) between 1st January and 1st May 2018. Twenty-four participants were included. Wet Cupping Therapy was applied to all individuals once every month for three months. Venous blood samples were collected before the first (Venousl) and after the last application (Venous 2) and cupping blood samples were collected during the first and last applications. Malondialdehyde (MAD) and total oxidant status (TOS) which both indicate oxidation; glutathione (GSH), superoxide dismutase (SOD), total antioxidant status (TAS), catalase (CAT) activities that indicate antioxidant effect, and protein levels were measured on the obtained serums. Results: The highest MDA and TOS levels and the lowest TAS, GSH, SOD and CAT levels were found in initial cupping blood. Significant improvement was observed in second cupping blood for these parameters when compared to initial values. We also found that MDA and TOS levels, as well as TAS, GSH, SOD and CAT levels, all changed favorably in the Venous 2 blood sample compared to the Venous 1 (p < 0.001). Conclusion: Wet cupping therapy seems a promising method for increasing antioxidant levels and curbing oxidative stress.Öğe The protective role of crocin in tartrazine induced nephrotoxicity in Wistar rats(Elsevier France-Editions Scientifiques Medicales Elsevier, 2017) Erdemli, Mehmet Erman; Gul, Mehmet; Altinoz, Eyup; Zayman, Emrah; Aksungur, Zeynep; Bag, Harika GozukaraThe present study was conducted to investigate the changes in rat kidney tissues after administration of tartrazine (T) and crocine (Cr). The latter was applied for its protective properties. The present study was conducted with the approval of Inonu University, Faculty of Medicine, Experimental Animals Ethics Committee. Forty rats were randomly divided into 4 equal groups (Control, T, Cr, T + Cr). At the end of the experiment, the rats were decapitated. Biochemical and histopathological studies were conducted on excised rat kidney tissues. It was determined that there was a significant increase in MDA, TOS, SOD, CAT, Bun, Creatinine levels in tartrazine administered rat kidney tissues for 21 days, while GSH and TAS levels decreased (P <= 0.05) when compared to all other groups. On the other hand, it was identified that Cr administration statistically significantly increased GSH and TAS levels in rat kidney tissues when compared to all other groups and decreased MDA and TOS levels to control group levels (P < 0.05). T group kidney sections exhibited different degrees of collapse in the glomeruli. In most sections, different levels of inflammatory cell infiltration and vascular and capillary congestion were detected in peritubular interstitial tissue. It was determined that T leads to adverse effects on rat kidney tissues. Administration of Cr + T prevented T induced nephrotoxicity. Thus, it was concluded that Cr could be utilized as a new type of anti-tartrazine toxicity agent.
