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Öğe Effects of cocoon incubation angle on hatching success of medicinal leeches (Hirudo spp.)(Taylor & Francis Ltd, 2023) Ceylan, Mustafa; Kucukkara, Ramazan; Karatas, EminProtocols for hirudiculture have not been fully established; many issues need to be investigated. The present study aimed to determine the effects of the cocoon incubation angle (CIA) on hatching success in hirudiculture, using leech species of Hirudo verbana. Cocoons were individually incubated in jars at three different angles, horizontally (0 & DEG;), angled (45 & DEG;), and vertically (90 & DEG;) for one month. The rot in cocoons, which was 17.5% in cocoons incubated horizontally, decreased gradually as the CIA increased, was 2.5% in cocoons incubated vertically. The average number of offspring obtained from cocoons increased as the CIA increased. The optimum CIA was determined to be 78.73 & DEG; and 60.63 & DEG; for the lowest rot in cocoons and the highest number of offspring, respectively. The present study revealed that although not statistically significant the CIA is a variable affecting the hatching success of medicinal leeches during the incubation period. This result is thought to be because the air ventilation inside the cocoons is more efficient in cocoons positioned at an angle or in a vertical position compared to cocoons incubated in a horizontal position. For profitable hirudiculture, we recommend that cocoons be positioned at an angle close to vertically, more than 60 & DEG; during incubation.Öğe Growth, survival and reproduction of the Turkish medicinal leech, Hirudo sulukii(Taylor & Francis Ltd, 2021) Ceylan, Mustafa; Kucukkara, Ramazan; Erbatur, Ismail; Karatas, Emin; Tunc, Meryem; Saglam, NaimThe present study aimed to investigate growth, survival and reproduction of the Turkish medicinal leech, Hirudo sulukii, endemic to Southeastern Anatolia and whose biology is poorly understood. In comparison with the Southern medicinal leech, Hirudo verbana, which has a wide distribution throughout Eurasia, H. sulukii grew faster until the fourth month, then growth increased significantly in favour of H. verbana. At the beginning of gravidity, high mortality occurred in specimens of H. sulukii not transferred to moist peat, the cocoon deposition area. Gravidity occurred in almost all (98%) H. sulukii adults, but was detected in only 60% of H. verbana adults. Unlike the growing period, survival rate was greater in H. sulukii during the reproductive period. Gravidity and cocoon laying periods lasted longer in H. sulukii, which continued reproductive activity even in colder conditions. There were more and smaller sized cocoons having higher deformation rate in H. sulukii. Likewise, fewer and larger offspring having lower morphological abnormality were observed in cocoons having lower hatchling rates in H. sulukii. Collectively, our findings reveal that H. sulukii reaches adult size relatively early, continues its reproduction in cold conditions and displays high reproductive performance, thus making it a strong candidate for leech aquaculture.