Yazar "Kurar, Ercan" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim Yok
    Öğe
    Expression of hypoxia-inducible factors and vascular endothelial growth factor during pregnancy in the feline uterus
    (Elsevier Science Inc, 2015) Agaoglu, Ozgecan Korkmaz; Agaoglu, Ali Reha; Guzeloglu, Aydin; Kurar, Ercan; Kayis, Seyit Ali; Ozmen, Ozlem; Schafer-Somi, Sabine
    Hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HIF gene family and VEGF in the cat uterus during pregnancy. Tissue samples of the whole uterine wall were collected after ovariohysterectomy and allocated to the following groups: embryo positive (group 1 [G1], n = 7, 7 days after mating), early pregnancy (group 2 [G2], n = 7, 20 days after mating), mid-pregnancy (group 3 [G3], n = 7, 24 days after mating), late pregnancy (group 4 [G4], n = 7,30-45 days after mating), and oocyte positive groups (group 5 [G5], n = 7, 7 days after induction of ovulation with GnRH analog). Relative mRNA levels were determined by real-time polymerase chain reaction. As housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase was used. The relative gene expression of HIF1A in G5 was found to be significantly higher than that of other groups (G1, G2, G3, and G4) (P < 0.05). In addition, the expression of HIF2A in G5 was higher than that of Cl and HIF2A gene expression at placentation sites of G4 was higher than in G1, G2, and G3 (P < 0.05). Immunohistochemistry indicated that HIF1A, HIF2A, and VEGF expressions were observed in different cell types of uterine and placental tissues in late pregnancy and oocyte groups. The expression of HIF3A did not change significantly in any group investigated. These observations suggest that HIFs and VEGF may play a role in the establishment and development of pregnancy. (C) 2015 Elsevier Inc. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Gene expression profiles of some cytokines, growth factors, receptors, and enzymes (GM-CSF, IFN?, MMP-2, IGF-II, EGF, TGF-?, IGF-IIR) during pregnancy in the cat uterus
    (Elsevier Science Inc, 2016) Agaoglu, Ozgecan Korkmaz; Agaoglu, Ali Reha; Guzeloglu, Aydin; Asian, Selim; Kurar, Ercan; Kayis, Seyit Ali; Schaefer-Somi, Sabine
    Early pregnancy is one of the most critical periods of pregnancy, and many factors such as cytokines, enzymes, and members of the immune system have to cooperate in a balanced way. In the present study, the gene expression profiles of factors associated with pregnancy such as EGF, transforming growth factor beta, granulocyte-macrophage colony stimulating factor, interferon gamma, insulin-like growth factor 2, insulin-like growth factor 2 receptor, and matrix metalloproteinase 2 were analyzed in uterine tissues of female cats. The cats were assigned to five groups: G1 (embryo positive, n = 7; 7th day after mating), G2 (after implantation, n = 7; 20th day after mating), G3 (midgestation, n = 7; 24-25th day after mating), G4 (late gestation, n = 7; 30-45th day after mating), G5 (oocyte group, n = 7; 7th day after estrus). Tissue samples from the uterus and placenta were collected after ovariohysterectomy. Relative messenger RNA levels were determined by real-time polymerise chain reaction. All the factors examined were detected in all tissue samples. In the course of pregnancy, significantly higher expression of EGF and matrix metalloproteinase 2 in G2 than in G1 was observed (P < 0.05). Insulin-like growth factor 2 expression was higher in all groups than in G1 (P < 0.05). Upregulation of EGF during implantation was detected. The expression of interferon gamma was significantly higher in G3 than in G1 (P < 0.05). Transforming growth factor beta and granulocyte macrophage colony-stimulating factor were constantly expressed in all groups. In conclusion; the expressions of these factors in feline uterine tissue at different stages of pregnancy might indicate that these factors play roles in the development of pregnancy such as trophoblast invasion, vascularization, implantation, and placentation. (C) 2016 Elsevier Inc. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Kısrak endometriyumunda phosphatase and tensin homolog (pten) geni mrna ekspresyonunun araştırılması
    (2015) Şen, Gonca; Hıtıt, Mustafa; Özel, Çaglayan; Güzeloglu, Aydın; Kurar, Ercan; Kayıs, Seyit Ali; Atlı, Mehmet Osman
    Amaç: Bu çalışmanın amacı, siklüs ve erken gebelik dönemlerinde dinamik bir yapıya sahip olan kısrak endometriyumunda phosphatase and tensin homolog (PTEN) gen ekspresyonunun mRNA düzeyinde belirlenmesidir.Gereç ve Yöntem: Araştırmada her güne 4 farklı kısrak olacak şekilde (n=4/gün) siklik kısraklardan ovulasyon gününde (d0), geç diöstrusta (LD) ve luteolizis sonrası östrusta (AL), gebe kısraklardan ise gebeliğin 14. (P14), 18. (P18) ve 22. (P22) günlerinde endometriyum biyopsi örnekleri toplandı. Doku örneklerinden total RNA elde edildi ve cDNA'ya dönüştürüldü. PTEN ekspresyonlarında siklus ve gebeliğe bağlı muhtemel değişiklikler mRNA seviyesinde kantitatif polimeraz zincir reaksiyonu (qPZR) kullanılarak araştırıldı. Referans gen olarak GAPDH ekspresyonu ile normalize edilen veriler karışık model kullanılarak analiz edildi. Farklı olan grup(lar) Asgari Önemli Fark (LSD) testi ile tespit edildi.Bulgular: PTEN ekspresyonu çalışmaya konu olan tüm siklüs ve erken gebelik dönemlerinde at endometriyumunda mRNA düzeyinde tespit edildi. LD'e göre AL'de ekspresyon seviyesinde anlamlı olmayan bir düşüş gözlendi. Benzer şekilde d0'a göre araştırılan P14, P18 ve P22 erken gebelik günlerinde PTEN ekspresyonunun baskılanmış olduğu tespit edildi. Ancak fark istatistiksel olarak anlamlı bulunmadı (P>0.10).Öneri: PTEN aktivitesinin ve protein düzeyinde ekspresyonun araştırılmasının anlamlı olacağı kanaatine varılmıştır.
  • Küçük Resim Yok
    Öğe
    Selection of reliable reference genes for qRT-PCR analysis on head and neck squamous cell carcinomas.
    (Scientific Publishers India, 2017) Yigin, Aysel Kalayci; Cora, Tulin; Acar, Hasan; Kurar, Ercan; Kayis, Seyit Ali; Colpan, Bahar; Ozturk, Kayhan
    The choice of reliable reference genes as an internal control is inevitable to obtain accurate results. Here we present an assessment of 7 reference genes (18S rRNA, 28S rRNA, ACTB, GAPDH, TUBA1, YWHAZ, and SDHA) to normalize gene expression data in Head and Neck Squamous Cell Carcinomas (HNSCCs). We attempted to determine a reliable set of reference genes to use in the normalization of gene expression data in Head and Neck Squamous Cell Carcinomas (HNSCCs) and normal mucosal tissues. Malignant and non-malignant tissue samples were collected from 12 patients with primary untreated HNSCC. geNorm and NormFinder software packages were used for data evaluations. Results obtained by geNorm indicated that average expression stability values (M) of all candidates genes were smaller than 1.5 (accepted M value for geNorm), showing that all the evaluated genes can be employed as HKGs, although GAPDH and ACTB were reported to be the most stable. Similarly, NormFinder results were in agreement with geNorm's results. GAPDH and ACTB are considered to be most suitable reference genes to evaluate novel gene expression in the tissues several of HNSCCs.