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    ANTIMICROBIAL ACTIVITY OF LUGOL SOLUTION AGAINST MICROORGANISMS ISOLATED FROM CLINICAL SPECIMENS
    (2023-05) Maqbol, Mustafa Ali Maqbol
    Both gram-positive and gram-negative bacteria benefit from iodine's antibacterial capabilities. However, bacterial infections are becoming increasingly difficult to treat as microorganisms develop resistance to traditional therapies, necessitating the progress of novel antibiotics. Developing new antibiotics represents a time-consuming process. As a result, the current study aims to assess the effectiveness of Lugol's solution as an antibacterial agent against antibiotic-resistant gram-negative and gram-positive bacteria obtained from clinical samples. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Lugol's solution are measured after completing antibiotic sensitivity tests on 100 bacterial isolates that were resistant to 70% or more of the tested antibiotics.These bacterial isolates were named strains with known antibiotic sensitivity and were used in the study. It was prepared that six serial dilutions of 2% Lugol's solution (32µl, 64µl, 128µl, 256µl, 512µl, 1024µl) and tested them on standard bacterial strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus). It was documented that the results of susceptibility, which measures the inhibition of bacterial growth. It was found that a concentration of 32µl of Lugol's solution did not inhibit any bacterial growth, while a concentration of 64µl inhibited 33.33% of the bacterial growth. At a concentration of 128µl, the inhibition rate increased to 66.66%, and at 256µl, the solution inhibited 100% of bacterial growth. Concentrations of 512µl and 1024µl also inhibited 100% of bacterial growth. It was also tested that 2% Lugol's solution on the 100 isolated bacterial specimens and found that the inhibition of bacterial growth increased steadily with increasing Lugol dilution. Specifically, a concentration of 32µl inhibited 9% of bacterial growth, and a concentration of 64µl inhibited 28% of bacterial growth. Concentrations of 128µl, 256µl, 512µl, and 1024µl inhibited 44%, 82%, 92%, and 96% of bacterial growth, respectively. Overall, gram-positive bacteria were more susceptible to Lugol's iodine solution than gram-negative bacteria. These results suggest that Lugol's solution may be useful in the treatment of bacterial infections, especially those caused by antibiotic-resistant strains, as an alternative to conventional antibiotics.