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    The effect of dimethoate on Capoeta capoeta (Guldenstaedt 1773) and the protective effect of ascorbic acid
    (Parlar Scientific Publications, 2016) Tanriverdi, E.A.; Yilmaz, M.; Koc, E.; Akgul, A.; Ersan, Y.
    In this study, Dimethoate (DM) (a pesticide)'s histopathological and electrophoretic effects were investigated on Capoeta capoeta (Guldenstaedt 1773) that were captured from Kars creek, and the protective effect of ascorbic acid (AA) (vitamin C) was tested. After allowing time for their adaptations to their new environment, the fish caught from Kars creek were divided into 4 groups (each including 10 fish), and placed in 300-liter tanks in the laboratory. The fish were kept in water containing different solutions: group I in normal water medium, group II in 100 mg/L AA, group III in 2 mg/L DM, and group IV in 100 mg/L AA and 2 mg/L DM. They were kept in these water mediums for 10 days. After this period, blood and tissue samples were taken from the fish for electrophoretic and histopathological studies. Tissue samples were detected in 10% formaldehyde solution and paraffin blocks were prepared by routine histological methods. The samples were cut into sections 3-5 ? thick, all stained according to hematoxylin and eosin staining methods, and they were examined under a microscope. Obtained serum samples were carried out in Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). When we compared the serum samples with the control group of fish, protein bands in the application of 100 mg/L AA and 2 mg/L DM, thickening in 36 kD band, thinning in 100 kD, 97 kD, 76 kD, 56 kD and 30 kD bands were observed, and the bands of 93 kD, 46 kD, 44 kD and 26 kD were detected as newly-synthesized in SDSPAGE electropherogram. In the histopathological examinations, depending on DM in intestine, liver, and kidney tissues obtained from the group formed by application of DM and AA, degeneration, necrosis, pyknotic nuclei and cell infiltrates were detected. As a result, whereas DM application was found to create toxic effects on Capoeta capoeta, AA was found to have a healing effect. © 2016 by PSP.
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    Efficiency of ascorbic acid against oxidative stress-induced dimethoate toxicity in fish, Capoeta capoeta [Guldenstaedt, 1773]
    (Triveni Enterprises, 2017) Yilmaz, M.; Kaya, I.; Koc, E.; Tanriverdl, E. A.; Kaya, M. M.; Ersan, Y.
    Aim: The main objective of the present study was to evaluate the effect of ascorbic acid against oxidative stress-induced by dimethoate in Capoeta capoeta. Methodology: The fish caught in Kars Creek were randomized in four equal groups and acclimatized in separate tanks. The fish were kept in tanks for 10 days as follows: Group I (control) was kept in normal water, Group II, Ill and IV were kept in separate tanks containing 2 mgrdimethoate and 2 mgr dimethoate plus 100 mgr' ascorbic acid and ascorbic acid. The level of total sialic acid, total antioxidant status and total oxidant status in fish serum were analyzed. Results: Total sialic acid level of Group III was found to be lower than the level of Group II (P< 0.05), there were no statistically important differences between these two groups in total antioxidant status and oxidant status. In gills, intensive cartilage degenerations and mononuclear cell infiltrations in group II and III were observed. It was found that toxic effects of dimethoate in group III decreased. Interpretation: Ascorbic acid in fish exposed to dimethoate reduced the level of total sialic acid and degenerations in lamellae of gills, it had no significant effect on the oxidative stress response. [GRAPHICS] .