Protective Effect of Allopurinol on Experimental Ovarian Ischemia-Reperfusion Injury Model of Rats

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dc.contributor.authorYurtcu, Engin
dc.contributor.authorTogrul, Cihan
dc.contributor.authorDeveci, Engin
dc.date.accessioned2024-09-29T16:12:17Z
dc.date.available2024-09-29T16:12:17Z
dc.date.issued2020
dc.departmentKarabük Üniversitesien_US
dc.description.abstractOBJECTIVE: To investigate the effect of allopurinol on an experimentally induced ovarian ischemia-repel fusion model. STUDY DESIGN: Female rats in the estrous cycle (n= 32) were divided into sham, ischemia, ischemia-reperfusion, and ischemia-reperfusion + allopurinol-treated groups. In the sham group the ovaries were opened and closed. In the ischemia group the ovaries were sealed for 2-hour ischemia. In the ischemia-reperfusion group, after ischemia, 2.5 hours of reperfusion was done. In the ischemia-reperfusion + allopurinol group, 3 hours after ischemia-reperfusion, 50 mg/kg allopurinol was administered. RESULTS: In the allopurinol-administered group, MDA levels were decreased. GSH values were decreased in the ischemia and ischemia-reperfusion group but increased in the allopurinol-treated group as compared to the control group. Caspase-3 expression was positive in enlarged corpus luteum cells. sFlt-1 expression was positive in vascular endothelial cells between preantral and antral follicles and some macrophages but negative in granular cells. In the ischemia group, sFlt-1 expression was positive in degenerative preantral and antral follicle cells, endothelial cells, and intense inflammatory cells. In the ischemia-reperfusion group, increased sFlt-1 expression was observed in luteal cells of the corpus luteum, vascular endothelial, and inflammatory cells. In the ischemia-reperfusion +allopurinol group, granular cells and corpus luteum cells showed decreased sFlt-1 expression, while being positive in vascular endothelial cells. CONCLUSION: Allopurinol inhibits development of apoptosis and reduces oxidative load in the ischemia-reperfusion stage, thus protecting the ovary from damage.en_US
dc.identifier.endpage16en_US
dc.identifier.issn0884-6812
dc.identifier.issue1en_US
dc.identifier.startpage8en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14619/8657
dc.identifier.volume42en_US
dc.identifier.wosWOS:000531823600002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSci Printers & Publ Incen_US
dc.relation.ispartofAnalytical and Quantitative Cytopathology and Histopathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectallopurinolen_US
dc.subjectCaspase-3en_US
dc.subjectFLT1 proteinen_US
dc.subjectfms-like tyrosine kinase-1en_US
dc.subjectischemiaen_US
dc.subjectischemia-reperfusion injuryen_US
dc.subjectovarian diseasesen_US
dc.subjectovarian ischemiaen_US
dc.subjectovarian torsionen_US
dc.subjectovaryen_US
dc.subjectoxidative stressen_US
dc.subjectratsen_US
dc.subjectreperfusion injuryen_US
dc.subjectsFlt-1en_US
dc.subjectvascular endothelial growth factor receptor-1en_US
dc.titleProtective Effect of Allopurinol on Experimental Ovarian Ischemia-Reperfusion Injury Model of Ratsen_US
dc.typeArticleen_US

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