Analysis of Molecular Resistance Mechanisms in Helicoverpa armigera (Hubner) (Noctuidae: Lepidoptera) Populations Under Pyrethroid Stress in Turkey

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dc.authoridKONUS, METIN/0000-0002-9953-1375
dc.contributor.authorKonus, Metin
dc.contributor.authorKaraagac, Sakine Ugurlu
dc.contributor.authorIscan, Mesude
dc.date.accessioned2024-09-29T16:11:17Z
dc.date.available2024-09-29T16:11:17Z
dc.date.issued2014
dc.departmentKarabük Üniversitesien_US
dc.description.abstractHelicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a major pest of economically important crops such as cotton, tomato and soybean. In order to control this pest, pyrethroid insecticides have been extensively used in farming areas all over the world. However, applications of excessive amounts of these insecticides can result in resistance development in the field populations of H. armigera. Resistance to the pyrethroids, beta-cypermethrin and lambda cyhalothrin, was analysed using bioassays. It was found that a canakkale field population of H. armigera field showed low (2.1-fold) and moderate (14.5-fold) resistance levels for beta-cypermethrin and lambda-cyhalothrin, respectively. Furthermore, expressions of selected CYP450, GST and esterase genes of H. armigera canakkale field populations were compared to those of a susceptible strain using real-time PCR. Our results indicate that H. armigera reacts to pyrethroids mainly by increasing expression levels of CYP450s such as CYP9Al2 and CYP9A14. However, GST and esterase genes expression levels were not significantly altered in a field population. GSTs and esterases were also analyzed using biochemical assays. While GSTs and esterase genes were not found to be up-regulated in the real-time PCR, except GST-DCNB activity, the biochemical assays also showed no significant increases in enzyme activities in the canakkale field population as compared to the susceptible strain. Consequently, CYP9Al2 and CYP9A14 together with certain GSTs, catalyzing DCNB substrate, are proposed to be involved in the metabolic responses against beta-cypermethrin and lambda-cyhalothrin insecticides in field population of H. armigera from Turkey.en_US
dc.description.sponsorship[METU-BAP-08-11-DPT2002-K120510]en_US
dc.description.sponsorshipWe thank Mr. R. Nauen (Bayer Crop Science AG) for susceptible H. armigera larvae samples. This study was also supported by the research fund: METU-BAP-08-11-DPT2002-K120510.en_US
dc.identifier.endpage92en_US
dc.identifier.issn1302-0250
dc.identifier.startpage81en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14619/8321
dc.identifier.volume16en_US
dc.identifier.wosWOS:000341280700009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGazi Entomological Research Socen_US
dc.relation.ispartofJournal of the Entomological Research Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHelicoverpa armigeraen_US
dc.subjectpyrethroid insecticidesen_US
dc.subjectqRT-PCRen_US
dc.subjectCYP450en_US
dc.subjectesterases and glutathione S-transferasesen_US
dc.titleAnalysis of Molecular Resistance Mechanisms in Helicoverpa armigera (Hubner) (Noctuidae: Lepidoptera) Populations Under Pyrethroid Stress in Turkeyen_US
dc.typeArticleen_US

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