N-glycosylation profiles of the green microalgae chlorella zofingiensis

dc.contributor.authorYaman, Mehmet Emrah
dc.contributor.authorAladag, Erdinç
dc.contributor.authorKayılı, Haci Mehmet
dc.contributor.authorKadıoglu, Yücel
dc.contributor.authorSalıh, Bekir
dc.date.accessioned2024-09-29T16:29:10Z
dc.date.available2024-09-29T16:29:10Z
dc.date.issued2021
dc.departmentKarabük Üniversitesien_US
dc.description.abstractNowadays, the use of microalgae species as raw materials in biopharmaceutical production is on the agenda. The reasonbehind this idea is that microalgae are cell factories that are able to efficiently utilize carbon dioxide for the production of numerous biologically active compounds. However, there are several problems that remain to be solved in the production of recombinant protein from microalgaes. One of the critical requirements is to produce a bio-compatible N-glycosylation profile from the secreted recombinant proteins. The knowledge about the glycosylation machinery and N-glycan profiles of microalgae spices are quite limited. In the study, it was aimed to characterize N-glycan profiles of a green microalgae, Chlorella zofingiensis. To achieve this, photoautotrophically grown Chlorella zofingiensis extracts including (glyco-)proteins were enzymatically deglycosylated and labelled with 2-aminobenzoic acid tag. Released N-glycans were purified with a HILIC-based approach and analyzed by MALDI-TOF(/TOF)-MS. The results showed that C. zofingiensis included oligomanno- sidic type N-glycan patterns. In addition, N-glycosylation profiles of C. zofingiensis by MALDI-MS revealed that most of the oligomannosidic N-glycans were phosphorylated.en_US
dc.identifier.doi10.15671/hjbc.717600
dc.identifier.endpage155en_US
dc.identifier.issn1303-5002
dc.identifier.issue2en_US
dc.identifier.startpage147en_US
dc.identifier.trdizinid483886en_US
dc.identifier.urihttps://doi.org/10.15671/hjbc.717600
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/483886
dc.identifier.urihttps://hdl.handle.net/20.500.14619/10319
dc.identifier.volume49en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofHacettepe Journal of Biology and Chemistryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleN-glycosylation profiles of the green microalgae chlorella zofingiensisen_US
dc.typeArticleen_US

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