Investigation of JC Virus Positivity By Real-time Polymerase Chain Reaction in Patients with Hematopoietic Stem Cell Transplant

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dc.authoridAltay Kocak, Aylin/0000-0002-0451-0142
dc.contributor.authorColak, Meryem
dc.contributor.authorKocak, Aylin Altay
dc.contributor.authorKaynar, Lale Aydin
dc.contributor.authorOzkurt, Zubeyde Nur
dc.contributor.authorYegin, Zeynep Arzu
dc.contributor.authorBozdayi, Gulendam
dc.date.accessioned2024-09-29T16:09:55Z
dc.date.available2024-09-29T16:09:55Z
dc.date.issued2020
dc.departmentKarabük Üniversitesien_US
dc.description.abstractIntroduction: In immunocompromised hosts, JC virus (JCV) can reactivate and cause a lytic infection of oligodendrocytes, resulting in progressive multifocal leukoencephalopathy (PML). Bone marrow is an important reservoir and possible site of neurotropic transformation for JCV. The aim of this retrospective study was to investigate the prevalance of JCV infection by real-time polymerase chain reaction (PCR) in patients sent from bone marrow transplant service to the laboratory in our hospital. Materials and Methods: A total of 153 clinical samples obtained from 62 patients with hematopoietic stem cell transplant between December 2013 and April 2018 were included into the study. Viral nucleic acids were extracted from the samples with QIAamp DSP Virus Kit in EZ1 Advanced (Qiagen, Germany) device. Isolated viral DNA was amplified with Real Star (R) JCV PCR Kit in Rotor-GeneQ (Altona, Germany) and JCV DNA was detected with qualitative method. Results: Sixty-two patients, 35 (56.5%) males and 27 (43.5%) females, between 18 years and 71 years of age were included into the study. Total JCV DNA positivity rate was found as 11.1% (17/153). Patients' diagnosis was respectively as follows: 45.2% acute myeloid leukemia, 19.4% acute lymphoblastic leukemia, 9.7% multiple myeloma. 6.4% myeloblastic sendrome, 6.4% non-Hodgkin lymphoma, 6.4% Hodgkin lymphoma, and 6.4% anemia. The distribution of JCV DNA positivity rates was found respectively as 40% acute myeloid leukemia, 30% multiple myeloma, 10% Hodgkin lymphoma, 10% acute lymphoblastic leukemia and 10% Non-hodgkin lymphoma. It was observed that 50% of JCV DNA positive patients died in the follow-up period after hematopoietic stem cell transplantation. Conclusion: It is not possible to diagnose JCV infections clinically because they are usually asymptomatic. However, up to 90% of those diagnosed with PML die within the first six months receiving a diagnosis. Detection and clinical surveillance JCV DNA by real-time PCR for hematopoietic stem cell transplantation patients is important for early diagnosis and treatment.en_US
dc.identifier.doi10.5578/flora.68466
dc.identifier.endpage46en_US
dc.identifier.issn1300-932X
dc.identifier.issue1en_US
dc.identifier.startpage40en_US
dc.identifier.trdizinid370566en_US
dc.identifier.urihttps://doi.org/10.5578/flora.68466
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/370566
dc.identifier.urihttps://hdl.handle.net/20.500.14619/7847
dc.identifier.volume25en_US
dc.identifier.wosWOS:000523303400006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isotren_US
dc.publisherBilimsel Tip Yayinevien_US
dc.relation.ispartofFlora Infeksiyon Hastaliklari Ve Klinik Mikrobiyoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectJC virusen_US
dc.subjectImmunosuppressive patienten_US
dc.subjectHematopoietic stem cell transplanten_US
dc.subjectReal-time polimerase chain reactionen_US
dc.titleInvestigation of JC Virus Positivity By Real-time Polymerase Chain Reaction in Patients with Hematopoietic Stem Cell Transplanten_US
dc.title.alternativeHematopoietik Kök Hücre Nakli Olmuş Hastalarda JC Virüs Pozitifliğinin Gerçek-Zamanlı Polimeraz Zincir Reaksiyonu ile Araştırılmasıen_US
dc.typeArticleen_US

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