Beneficial role of crocin against doxorubicin-induced testicular damage in rats: insights into vimentin modulation

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dc.authoridOzgul Onal, Melike/0000-0001-6710-5729
dc.authoridAltinoz, Eyup/0000-0002-3991-9773
dc.contributor.authorozgul-onal, Melike
dc.contributor.authorAljumaili, Sara Asaad Abdulkareem
dc.contributor.authorYigitturk, Gurkan
dc.contributor.authorYasar, Volkan
dc.contributor.authorBicer, Yasemin
dc.contributor.authorCetinavci, Dilan
dc.contributor.authorAltinoez, Eyup
dc.date.accessioned2024-09-29T16:06:33Z
dc.date.available2024-09-29T16:06:33Z
dc.date.issued2024
dc.departmentKarabük Üniversitesien_US
dc.description.abstractPurpose: Doxorubicin (DOX) is a wide-spectrum antibiotic used for chemotherapy. Its side effects limit treatment. Crocin is one of the carotenoids that has both anti-inflammatory and antioxidant activities. We aimed to evaluate the effects of crocin against doxorubicin-induced testicular damage in rats. Materials and Methods: Forty Wistar rats were divided into four groups. Group 1: Control, Group 2: Crocin, Group 3: DOX, Group 4: DOX+Crocin (n=10, for all). Testis tissues were stained with Hematoxylin-Eosin. The diameters of seminiferous tubules were measured and the testicular mean histopathologic damage score (MHDS) was calculated. Vimentin expression in Sertoli cells was calculated as H-Score. Levels of Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT), and Superoxide dismutase (SOD) activities were determined in testis tissues. Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) were also calculated. Results: Atrophic seminiferous tubules were seen in the DOX group. Edema, vacuolization, and disorganization were present in the injured tubules. The MHDSs for the DOX group and control groups were 4.60 +/- 0.45 and 0.20 +/- 0.13, respectively. Both of these groups showed a significant difference. The histopathologic score was reduced after using crocin. Tubule damage considerably decreased while immunoexpression levels of vimentin and seminiferous tubule width significantly increased in the DOX+Crocin group compared to the DOX group. MDA and TOS levels were significantly increased after DOX treatment, and GSH, SOD, CAT, and TAS levels were significantly decreased. All biochemical indicators were greatly improved after receiving crocin. Conclusion: Crocin supplementation exhibited adequate beneficial effects against the testicular damage of DOXinduced function by balancing the oxidant/antioxidant status.en_US
dc.description.sponsorshipKarabuk University Scientific Research Fund [TYL-2020-2298]en_US
dc.description.sponsorshipThe research was supported Karabuk University Scientific Research Fund (TYL-2020-2298) .en_US
dc.identifier.doi10.17826/cumj.1420141
dc.identifier.endpage342en_US
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue2en_US
dc.identifier.startpage332en_US
dc.identifier.trdizinid1248681en_US
dc.identifier.urihttps://doi.org/10.17826/cumj.1420141
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1248681
dc.identifier.urihttps://hdl.handle.net/20.500.14619/6905
dc.identifier.volume49en_US
dc.identifier.wosWOS:001292718000001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherCukurova Univ, Fac Medicineen_US
dc.relation.ispartofCukurova Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject. . Doxorubicinen_US
dc.subjectcrocinen_US
dc.subjecttestisen_US
dc.subjectvimentinen_US
dc.subjectoxidative stressen_US
dc.titleBeneficial role of crocin against doxorubicin-induced testicular damage in rats: insights into vimentin modulationen_US
dc.typeArticleen_US

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