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Öğe Determination of leaf and stomata characteristics of some late blooming almond (amygladus communis l.) Cultivars and their hybrids grown in semi-arid climate conditions(2023) Ak, Bekir Erol; Can, Mesut; Açar, İzzet; Hatıpoglu, İbrahim Halil; Dıkmetaş, BirgülThis study was carried out on some domestic and foreign late blooming varieties and their genotypes obtained as a result of hybridization in the almond collection parcel of Harran University Faculty of Agriculture in Sanliurfa/Turkiye in the summer period of 2020. In the research, 5 different cultivars and 6 hybrids were examined in the orchard. In the study, 198 leaves were taken from 3 trees of each variety and 9 of each tree from 2 directions. It was planned and carried out according to the split plots experimental design in random blocks. North and South directions were taken into account when taking leaf samples. According to the findings, when the leaf characteristics were examined in general, the highest values in terms of leaf width, leaf length, petiole length and leaf area were found in ‘Genotype-7’ hybrids among cultivars and hybrids. As a result of stoma analysis on the lower surface of the leaves, the highest overall average (196.47 units mm-²) was determined in Genotype-3, while the lowest (127.10 units/mm²) was found in ‘Ferragnes’ variety. As a result of the analysis, when all the average values were taken into account, it was determined that the leaf area was 16.74 cm², the average stomata density was 153.51/mm² and the leaf area was 256975.74/leaf stomata. It is thought that the study will be descriptive for almond varieties and hybrids grown in the same ecology. This study was carried out in order to determine the effects of the adaptation of the almond varieties that are intensively grown in the Southeastern Anatolia Region and that bloom late, and some hybrids that are likely to be grown in the region in the future, on the morphological characteristics.Öğe Optimization of in vitro sterilization for pistachio (pistacia vera l.) Rootstocks(2022) Korkmaz, Ecenur; Yaşar, Ramazan; Yaşar, Büşra; Sarpkaya, Kamil; Açar, İzzet; Asım, MuhammadPistachio (Pistacia vera L.) is one of the leading edible nut consumed all over the World due to its nutritional values. The plant is cultivated in most of the countries alongwith Turkey which is one of the the leading grower of pistachio. In Turkey, the rootstock material is currently propagated through traditional methods and there is a need of propagating plant material using modern biotechnological techniques like plant tissue culture. The provision of contaminated free explants with minimum or no phenolic compounds in the culture medium is the prerequisite of in vitro regeneration protocol. The plant material used in this study was collected at different physiological stages during different months like April- June and Sep-October. The plant material was cut into 2-3 cm long nodal segments followed by cleaning with different agents like water, soap and fungicide prior to subjected to sterilizing agents. Different sterilizing agents used in this study were HgCl2, Huwa-san (H2O2) and commercial bleach (NaOCl) for both rootstocks (UCB-1 and Buttum) with different exposure time. Sterilized explants were cultured on MS basal medium containing plant growth regulators and subcultured once a week for three weeks. Results revealed that HgCl2 as sterilizing agent was more superior than other sterilizing agents for both rootstocks. Among rootstocks, UCB-1 was more responsive than Buttum and relatively more sterilized plants were attained. On the other hand, plant material collected during June responded better and 90.0% and 50.0% sterilzied plants were attained for UCB-1 and Buttum respectively. The results revealed the significant impact of collection time, sterilizing agent type, concentration and exposure time on sterilization of P. vera rootstocks.