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Öğe Bactericidal and in vitro osteogenic activity of nano sized cobalt-doped silicate hydroxyapatite(Elsevier Sci Ltd, 2022) Alshemary, Ammar Z.; Hussain, Rafaqat; Dalgic, Ali Deniz; Evis, ZaferHydroxyapatite (HA) particles with enhanced antibacterial properties can be prepared by integrating metal ions into the crystal structure of the nanoparticles. Cobalt and silicate ions containing HA (Co/Si-HA) with the formula Ca10-xCox(PO4)(6-y)(SiO4)(y)(OH)(2) (x = 0.2, 0.6, and 1.0 and y = 0.5) was successfully synthesised by using microwave-assisted wet precipitation method. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and inductively coupled plasma mass spectrometry (ICP-MS) techniques were used to characterise the synthesised nanoparticles. The results revealed that the incorporation of SiO44- ions increased the lattice parameters and decreased the crystallite size of HA. However, the incorporation of Co(2+)ions led to the reduction of lattice parameters and the particle size of the SiHA nanoparticles. In vitro antibacterial activity of materials was evaluated using disk diffusion and minimum inhibitory concentration (MIC) protocols. The findings indicated that incorporating Co2+ ions into SiHA inhibited the growth of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus). The cytotoxicity of materials evaluated using the Sarcoma osteogenic (Saos-2) cell line revealed that they were cytocompatible and exhibited no adverse side effects. The osteogenic differentiation of cells was confirmed by the significant increase in the alkaline phosphatase (ALP) activity by incorporating Co2+/SiO44- ions into the HA crystal structure. Our results show that the nanoparticles prepared in this study have a promising future in biomaterial-tissue engineering applications.Öğe Physico-chemical and biological properties of hydroxyapatite extracted from chicken beaks(Elsevier Science Bv, 2018) Alshemary, Ammar Z.; Akram, Muhammad; Taha, Ali; Tezcaner, Aysen; Evis, Zafer; Hussain, RafaqatIn this investigation, we have extracted biological hydroxyapatite (HA) containing magnesium, sodium, aluminium, zirconium and silicon ions from the chicken beaks. Raw chicken beaks were calcined at different temperatures after washing with boiled water and organic solvents. The calcined biological HA was characterized by X-ray diffraction (XRD), Fourier transforms infrared (FTIR), Field emission electron microscopy (FESEM), X-ray fluorescence (XRF) spectroscopy and Thermogravimetric analysis (TGA). The viability of Saos-2 cells treated with extracts of biological HA was higher than cells on tissue culture plates (TCPs) and synthetic HA, suggesting a good cytocompatibility of biological HA. Our research has successfully shown that the chicken beaks are a cheap source of biological HA. (C) 2017 Elsevier B.V. All rights reserved.Öğe Porous clinoptilolite-nano biphasic calcium phosphate scaffolds loaded with human dental pulp stem cells for load bearing orthopedic applications(Iop Publishing Ltd, 2019) Alshemary, Ammar Z.; Pazarceviren, Ahmet Engin; Keskin, Dilek; Tezcaner, Aysen; Hussain, Rafaqat; Evis, ZaferClinoptilolite (Cpt)-nanohydroxyapatite (HA) (Cpt-HA) scaffolds were fabricated as a potential material for load bearing orthopaedic applications. Cpt-HA materials were successfully synthesized by using microwave assisted reflux method followed by the fabrication of three-dimensional (3D) porous scaffold via thermal decomposition process using polyethylene glycol (PEG)/polyvinyl alcohol (PVA) as porogens. The scaffold materials were characterized using x-ray diffraction, Fourier transform Infra-red, Scanning electron microscopy and Energy dispersive spectroscopy techniques. Incorporation of Cpt in HA scaffold significantly increased the compressive strength and surface hardness while scaffolds retained an interconnected porous structure with 64% porosity. Human dental pulp stem cells (DPSCs) were isolated from the third molar and used as pluripotent-like cell model to evaluate the biological properties of Cpt-HA scaffolds. Highest cellular attachment and proliferation were observed for DPSCs seeded on 2.0 g Cpt-HA scaffolds compare to pure HA. Similarly, significantly higher ALP activity of cells was observed on Cpt-HA scaffolds compared to pure HA. The enhanced proliferation and osteogenic response of the DPSCs cultured on Cpt-HA scaffolds suggest that the fabricated scaffolds can be used in bone tissue engineering. In this work, we have successfully shown that the interconnected porous Cpt-HA scaffolds have superior mechanical biological properties compared to pure HA scaffold.Öğe Strontium doped injectable bone cement for potential drug delivery applications(Elsevier Science Bv, 2017) Taha, Ali; Akram, Muhammad; Jawad, Zaidoon; Alshemary, Ammar Z.; Hussain, RafaqatMicrowave assisted wet precipitation method was used to synthesize calcium deficient strontium doped beta-tricalcium phosphate (Sr-beta TCP) with a chemical formula of Ca2.96-xSrx(PO4)(2). beta-FICP was reacted with monocalcium phosphate monohydrate [Ca(H2PO4)(2)center dot H2O, MCPM] in presence of water to furnish corresponding Sr containing brushite cement (Sr-Brc). The samples were characterized by using X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). Strontium content in the prepared samples was determined by using inductively coupled plasma optical emission spectrometry (ICP-OES). The effect of Sr2+ ions on the structural, mechanical, setting properties and drug release of the cement is reported. Incorporation of Sr2+ ions improved the injectability, setting time and mechanical properties of the Brc The release profiles of antibiotics incorporated in Brc and Sr-Brc confirmed that the Sr incorporation into the Brc results in the efficient release of the antibiotics from the cement. (C) 2017 Elsevier B.V. All rights reserved.
