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Öğe Cytotoxic effect of extract from Dunaliella salina against SH-SY5Y neuroblastoma cells(General Physiol And Biophysics, 2015) Ataseyer-Arslan, Belkis; Yilancioglu, Kaan; Bekaroglu, Maide G.; Taskin, Emre; Altinoz, Eyup; Cetiner, SelimCytotoxic effects of essential oils extracted from Dunaliella sauna on SH-SY5Y human neuroblastoma cells were investigated in this study. GC-MS analysis was used for determination of the composition of essential oils found in Dunaliella salina extract. All experimented concentrations of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells were significantly more cytotoxic than the tested concentrations of the extract on ECV304 human endothelial cells used as a control. Fifthy compounds were detected in GC-MS analysis of the extract, and five major compounds were predominantly found as follows: octadecanoic acid, methyl ester (27.43%); hexadecanoic acid, methyl ester (Cas) methyl palmitate (24.82%); 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (7.39%); octadecanoic acid (5.03%), pentadecanoic acid (3.60%). The cytotoxic activity of Dunaliella salina extract on SH-SY5Y human neuroblastoma cells might be due to high concentrations of octadecanoic acid and hexadecanoic acid. Furthermore, results indicate that the extract demonstrates some proliferative effect on ECV304 cells in a dose-dependent manner between 0.25 and 5 mu g/ml. These results suggest that Dunaliella sauna may have anticancer potential against human neuroblastoma cells.Öğe THE EFFECT OF SAFFRON (ITS ACTIVE CONSTITUENT, CROCIN) ON THE CARDIOVASCULAR COMPLICATION AND DYSLIPIDEMIA IN STREPTOZOTOCIN INDUCED DIABETIC RATS(African Networks Ethnomedicines, 2015) Altinoz, Eyup; Taskin, Emre; Oner, Zulal; Elbe, Hulya; Arslan, Belkis AtaseverBackground: Diabetes mellitus (DM) causes serious complications such as coronary heart disease, atherosclerosis, nephropathy, retinopathy and neuropathy. Materials and Methods: Rats were randomly divided into three groups, each containing 10 rats: control group, DM group; DM+crocin group. Normal saline was administered in the control and DM groups, and crocin was administered in DM+crocin group at a dose of 20 mg/kg bw/day for 21 days. Trunk blood and the hearth tissue were collected for histopathological and biochemical examination. Results: DM led to increment of MDA levels (p<0.05) and the serum levels of total cholesterol, TG, LDL and VLDL (p<0.05) and decrease of GSH levels (p<0.01) compared to control group in serum and heart tissue. Diabetic rats were treated with crocin, MDA levels (p<0.05) and the levels of total cholesterol, TG and VLDL decreased significantly, but GSH levels (p<0.05) increased remarkably compared to DM rats. Statistically significant increase in histopathological damage score was found in the DM group (p<0.0001). Histopathological changes markedly regressed in DM+crocin group. Conclusion: Our results showed that crocin might prevent diabetes induced cardiovascular complications by reduction of oxidative stress and dyslipidemia.Öğe Evaluation of ACE2 and AT2R Polymorphisms in Patients with Asthma(Wiley-Blackwell, 2015) Ayada, Ceylan; Toru, Umran; Genc, Osman; Sahin, Server; Taskin, Emre; Yasar, Zehra; Bulut, Ismet[No abstract available]Öğe Evaluation of multidrug resistance-1 gene C > T polymorphism frequency in patients with asthma(Hospital Clinicas, Univ Sao Paulo, 2015) Toru, Umran; Ayada, Ceylan; Genc, Osman; Yasar, Zehra; Sahin, Server; Taskin, Emre; Bulut, IsmetOBJECTIVES: Asthma is a chronic inflammatory lung disease characterized by bronchial hyperresponsiveness and airflow obstruction. Genetic and oxidative stress factors, in addition to pulmonary and systemic inflammatory processes, play a pivotal role in the pathogenesis of asthma. The products of the multidrug resistance-1 gene protect lung tissue from oxidative stress. Here, we aimed to evaluate the association between the multidrug resistance-1 gene C>T polymorphism and asthma with regard to oxidative stress-related parameters of asthmatic patients. METHODS: Forty-five patients with asthma and 27 healthy age-matched controls were included in this study. Blood samples were collected in tubes with ethylenediaminetetraacetic acid. DNA was extracted from the blood samples. The multidrug resistance-1 gene polymorphism was detected by polymerase chain reaction and a subsequent enzyme digestion technique. The serum levels of total oxidant status and total antioxidant status were determined by the colorimetric measurement method. RESULTS: The heterozygous polymorphic genotype was the most frequent in both groups. A significant difference in the multidrug resistance-1 genotype frequencies between groups indicated an association of asthma with the TT genotype. A significant difference between groups was found for wild type homozygous participants and carriers of polymorphic allele participants. The frequency of the T allele was significantly higher in asthmatic patients. The increase in the oxidative stress index parameter was significant in the asthma group compared with the control group. CONCLUSIONS: The multidrug resistance-1 gene C/T polymorphism may be an underlying genetic risk factor for the development of asthma via oxidant-antioxidant imbalance, leading to increased oxidative stress.Öğe The influence of RS738409 I148M polymorphism of patatin-like phospholipase domain containing 3 gene on the susceptibility of non-alcoholic fatty liver disease(Lippincott Williams & Wilkins, 2021) Akkiz, Hikmet; Taskin, Emre; Karaogullarindan, Umit; Delik, Anil; Kuran, Sedef; Kutlu, OzlemWe aimed to elucidate the frequency of polymorphic genotypes and alleles of patatin-like phospholipase domain containing 3 rs738409 polymorphism and its possible associations with non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis in a cohort from Turkey. We enrolled 200 patients diagnosed with NAFLD and genotyped for rs738409 I148M polymorphism by real-time polymerase chain reaction, particularly by melting curve analysis. SPSS analysis software was used for statistical significance. Continuous variable values were expressed as mean +/- standard deviation. Significant statistical level was chosen as p = 0.05. Our results demonstrate in a cohort from Turkey that rs738409 C > G polymorphism (I148M) of patatin-like phospholipase domain containing 3 gene is significantly able to affect individuals to have NAFLD in unadjusted regression model. Consistent with the previous studies in other populations, our study group showed a significantly higher risk of having NAFLD in unadjusted regression model but not in the adjusted model indicating that non-genetic factors such as age and sex may be responsible for the association. However, independent studies need to validate our findings with a larger group of NAFLD patients, as well as in different ethnic cohorts.Öğe Investigation of associations between apolipoprotein A5 and C3 gene polymorphisms with plasma triglyceride and lipid levels(Assoc Medica Brasileira, 2023) Taskin, Emre; Bagci, Hasan; Turan, Muhammed KamilOBJECTIVE: The aim of this study was to determine frequency and associations between APOA5 c.56C>G, -1131T>C, c.553G>T, and APOC3-482C>T and SstI gene polymorphisms with hypertriglyceridemia. METHODS: Under a case-control study model, 135 hypertriglyceridemic and 178 normotriglyceridemic control participants were recruited. Polymerase chain reaction and restriction fragment length polymorphism methods were utilized for genotyping. Statistical calculations were performed by comparing allele and genotype frequencies between groups. Clinical characteristics were compared between groups and intra-group genotypes. RESULTS:APOC3 gene -482C>T and SstI polymorphic genotypes and allele frequencies were significantly higher in hypertriglyceridemic group (genotype frequencies, p=0.035, p=0.028, respectively). Regression analysis under unadjusted model confirmed that APOC3-482C>T and SstI polymorphisms were significantly contributing to have hypertriglyceridemia (p=0.02, odds ratio [OR]=1.831 (95% confidence interval [CI] 1.095-3.060); p=0.04, OR=1.812 (1.031-3.183), respectively).APOA5 c.56C>G was in complete linkage disequilibrium with APOA5 c.553G>T polymorphism (D'=1). CONCLUSION: For the first time in a population sample from Turkey, among the five polymorphisms of APOA5 and APOC3 genes investigated,APOC3-482C>T and SstI polymorphisms were associated with elevated serum TG levels, while APOA5 c.56C>G, -1131T>C, and c.553G>T polymorphisms were not.Öğe Lack of Association Between Interleukin 6 Gene Promoter Polymorphisms and Aneurysmal Subarachnoid Hemorrhage in Turkish Population(Journal Neurological Sciences, 2015) Bayri, Yasar; Taskin, Emre; Ulus, Aykan; Bayrakli, Fatih; Altun, Adnan; Bagci, HasanBackground: The IL6 gene is related to several disease states. Its relation to aneurysm formation and aneurysmal SAH has been studied in the literature, but the reported results have not resulted in definite positive or negative data. In this study we aimed to examine IL6 gene polymorphisms in a Turkish population and further our understanding of the relationship. Results: We included 120 intracranial aneurysm SAH cases and 120 healthy controls to examine whether there was a genotype difference between these groups in the -174 G > C and -572 G > C promoter polymorphisms located in the IL6 gene. The differences between groups were studied using a chi-squared test. There was no statistical difference between aneurysmal SAH patients and controls for IL6 promoter 174G > C and 572G > C polymorphisms. Conclusions: IL6 promoter SNPs did not show a correlation with intracranial aneurysm formation and subarachnoid aneurysmal rupture in a Turkish population, but genetic differences between ethnic populations may give different results.Öğe Smoking prevalence, associated attitudes and comparison of negative automatic thoughts among high school students in Turkey(European Respiratory Soc Journals Ltd, 2015) Acat, Murat; Memis, Cagdas Oyku; Turan, Muhammet Kamil; Benli, Ali Ramazan; Taskin, Emre; Yasar, Zehra; Memis, Seda Derici[No abstract available]
