Yazar "Yilmaz, Nesibe" seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Effects of the genetic variants and serum levels of TIMP-1 and TIMP-2 on type 2 diabetes mellitus in the Turkish population(Bayrakol Medical Publisher, 2024) Celik, Faruk; Yilmaz, Umit; Yilmaz, Nesibe; Ozyavuz, Kerem; Basaran, Cem; Fazliogullari, Osman; Ergen, ArzuAim: Genetic and environmental factors are very important in the formation of type 2 diabetes mellitus (T2DM). Tissue inhibitors of matrix metalloproteinases (TIMPs) play central roles in inhibition of the extent of extracellular matrix degradation. The aim of this study is to investigate serum levels and gene polymorphisms of TIMP-1 and TIMP-2, and their effects on T2DM in the Turkish population. Material and Methods: One hundred seventeen patients with T2DM and 127 healthy controls were included in this study. TIMP-1 372 T>C, TIMP-2 303C>T, and TIMP-2 418 G>C polymorphisms were determined by PCR-RFLP method and serum TIMPs levels were measured by ELISA. Results: The frequencies of the TT genotype and T allele of the TIMP-2 303 C>T polymorphism were significantly higher in the patient group than in the control group. The frequency of the C allele for TIMP-2 418 G>C polymorphism was significantly higher in the control group than in patients. TIMP-1 372 T>C polymorphism was not statistically significant between patients and controls. Additionally, TIMP-1 serum levels were statistically higher in T2DM patients than in controls. Discussion: This study provides the first evidence that the TT genotype and T allele of the TIMP-2 303 C>T polymorphism significantly contribute to the risk of T2DM in the Turkish population. Also, carrying the C allele of the TIMP-2 418 G>C polymorphism had a protective effect against the development of T2DM. In addition, our results suggest that the C allele of the TIMP-1 372 T>C polymorphism may have protective effects against the development of T2DM.Öğe Intracerebroventricular injection of spexin stimulates the hypothalamic-pituitary-testicular axis and increases the secretion of male reproductive hormones in rats(Elsevier Gmbh, 2024) Yilmaz, Nesibe; Ibrahim, Rida Zahiraldin; Yildiz, AzibeBackground: Male reproductive functions are regulated in the hypothalamic-pituitary-gonadal (HPG) axis. Any problem in this axis would lead to the deterioration of reproductive functions. The present study aimed to investigate the effects of intracerebroventricular (icv) Spexin (SPX) infusion on the HPG axis in detail. Methods: 40 Wistar albino rats were divided into four groups: control, sham, SPX 30 nmol and SPX 100 nmol (n=10). 30 nmol/1 mu l/hour SPX was administered icv to the rats in the SPX 30 nmol group for 7 days, while rats in the SPX 100 nmol group were administered 100 nmol/1 mu l/hour SPX. On the 7th day, the rats were decapitated, blood and tissue samples were collected. Serum LH, FSH and testosterone levels were determined with the ELISA method, GnRH mRNA expression level was determined in hypothalamus with the RT-PCR method. Seminiferous tubule diameter and epithelial thickness were determined with the hematoxylin-eosin staining method. Results: SPX infusion was increased GnRH mRNA expression in the hypothalamus tissue independent of the dose (p<0.05). Serum LH, FSH and testosterone levels in the SPX groups were increased when compared to the control and sham groups independent of the dose (p <0.05). Histological analysis revealed that SPX infusion did not lead to any changes in seminiferous epithelial thickness, while the tubule diameter increased in the SPX groups (p<0.05). Conclusion: The study findings demonstrated that icv SPX infusion stimulated the HPG axis and increased the secretion of male reproductive hormones.Öğe Intracerebroventricular PROK2 infusion could increase the secretion of male reproductive hormones by stimulating the HPG axis(Springer, 2024) Yilmaz, Nesibe; Yildiz, AzibeBackgroundProkineticin 2 (PROK2), an important neuropeptide that plays a key role in the neuronal migration of gonadotropin-releasing hormone (GnRH) in the hypothalamus, is known to have regulatory effects on the gonads. In the present study, the impact of intracerebroventricular (icv) PROK2 infusion on hypothalamic-pituitary-gonadal axis (HPG) hormones, testicular tissues, and sperm concentration was investigated.Methods and resultsRats were randomly divided into four groups: control, sham, PROK2 1.5 and PROK2 4.5. Rats in the PROK2 1.5 and PROK2 4.5 groups were administered 1.5 nmol and 4.5 nmol PROK2 intracerebroventricularly for 7 days via an osmotic mini pump (1 mu l/h), respectively. Rat blood serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone hormone levels were determined with the ELISA method in the blood samples after 7 days of infusion. GnRH mRNA expression was determined with the RT-PCR in hypothalamus tissues. analyze Sperm concentration was determined, and testicular tissue was examined histologically with the hematoxylin-eosin staining method. It was observed that GnRH mRNA expression increased in both PROK2 infusion groups. Serum FSH, LH and testosterone hormone levels also increased in these groups. Although sperm concentration increased in PROK2 infusion groups when compared to the control and sham, the differences were not statistically significant. Testicular tissue seminiferous epithelial thickness was higher in the PROK2 groups when compared to the control and sham groups.ConclusionThe present study findings demonstrated that icv PROK2 infusion induced the HPG axis. It could be suggested that PROK2 could be a potential agent in the treatment of male infertility induced by endocrinological defects.Öğe Investigation of the Effect of Astaxanthin on Autophagy in Renal Ischemia-reper fusion Modeled Rats(Galenos Publ House, 2024) Kisaoglu, Aysegul; Kose, Evren; Yilmaz, Nesibe; Tanbek, Kevser; Yildiz, Azibe; Yilmaz, Umit; Cirik, Rumeyza HilalObjective: The aim of this study was to investigate the effect of various astaxanthin (ATX) doses on oxidative damage and autophagy in renal ischemia-reperfusion (I/R) injury-modeled rats. Methods: The rats were divided into five groups: sham group (n=8), I/R (n=8), I/R + 5 mg/kg ATX (n=8), I/R + 10 mg/kg ATX (n=8), and I/R + 25 mg/ kg ATX (n=8) groups. ATX was dissolved in 5 mg/kg, 10 mg/kg, and 25 mg/ kg olive oil for 7 days and administered to the rats in the experimental group. Sham and I/R groups were also administered ATX solution (olive oil) via oral gavage for 7 days. Renal ischemia reperfusion was induced in all rats except the sham group after the last dose was administered on the 7 th day. Reperfusion was conducted for 24 hours after 45 minutes of ischemia. Results: Blood samples were collected, and kidney tissue were incised for biochemical and histological analyses. Superoxide dismutase (SOD) and total antioxidant status (TAS) were significantly lower in the I/R group than in the sham group (p<0.05), whereas malondialdehyde (MDA) and total oxidant status (TOS) values were higher (p<0.05). It was determined that SOD and TAS increased and MDA and TOS decreased in the ATXadministration groups compared with the I/R group, independent of the dose (p<0.05). In the 25 mg/kg ATX + I/R group, Beclin-1 and LC3 0 immunoreactivities were significantly higher than those in the other groups (p<0.05). The lowest p62 immunoreactivity was observed in the 25 mg/kg ATX + I/R group. Conclusions: ATX had a protective effect on kidney function and against oxidative damage. Furthermore, high-dose ATX administration protected kidney tissue via autophagy induction in this study.Öğe Investigation of the Effect of Astaxanthin on Oxidative Stress in Renal Ischemia-Reperfusion Modeled Rats(Wiley, 2022) Kisaoglu, Aysegul; Kose, Evren; Yilmaz, Nesibe; Tanbek, Kevser; Yilmaz, Umit; Ozbag, Davut[No abstract available]Öğe Phoenixin-14 may ameliorate testicular damage caused by torsion-detorsion by reducing oxidative stress and inflammation in prepubertal rats(Churchill Livingstone, 2024) Yilmaz, Nesibe; Hudaykuliyeva, Jemal; Gul, SemirThe present study aimed to investigate the effects of Phoenixin-14 (PNX-14) on oxidative damage, inflammatory response, histopathological variations, and serum testosterone levels in testicular tissues. Forty-eight Wistar albino prepubertal male rats were divided into 4 groups (Sham, TTD, TT +PNX +TD, TTD +PNX) (n =12). The torsion period was 2 hours and the detorsion period was 24 hours in the testicular torsion/detorsion (TD) groups. A single PNX-14 (50 mu g/kg) dose was injected into the rats in the TT +PNX TD group on the 90th minute of torsion, and it was injected into the rats in the TTD +PNX group at the beginning of detorsion. Oxidative damage in testicular tissues was determined based on superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS), and inflammatory damage was determined based on TNF- alpha and IL -6 levels. Histopathological variations were investigated with the Periodic Acid Schiff (PAS) staining method in testicular tissues and analyzed based on Johnsen scores. Spermatogonia cells were examined immunohistochemically. Serum testosterone levels were determined with the enzyme -linked immunosorbent assay (ELISA). A significant increase in oxidative stress and inflammation parameters was determined in the TTD group when compared to the other groups (p <0.05). PNX-14 treatment led to a statistically significant decrease in these parameters and significantly repaired the TD damage in testicular tissue (p <0.05). Johnsen scoring revealed significant improvement in PNX-14 groups and an increase in spermatogonia count, supporting the biochemical findings (p <0.05). PNX-14 could be a potential therapeutic agent in testicular TD damage and further studies should be conducted to elucidate the present study findings.Öğe Pinealectomy and melatonin administration in rats: their effects on pulmonary edema induced by ?-naphthylthiourea(Taylor & Francis Ltd, 2023) Al Gburi, Mohammed Raed Abdullah; Altinoz, Eyup; Elbe, Hulya; Onal, Melike Ozgul; Yilmaz, Umit; Yilmaz, Nesibe; Karayakali, MelikeWe aimed to observe the possible effects of melatonin (MLT) deprivation (pinealectomy) and exogenous MLT administration on pulmonary edema induced by alpha-naphthylthiourea (ANTU), a toxic chemical agent, in rats. Seventy animals were assigned to seven groups: control, sham pinealectomy (PINX), PINX, ANTU (10 mg/kg intraperitoneal on day 30), ANTU + MLT (10 mg/kg/day i.p. for 30 days), ANTU + PINX, and ANTU + PINX + MLT. In this study, pleural effusion (PE) formation, lung weight/body weight (LW/BW) and PE/BW ratios (fluid accumulation and weight values in the lungs) increase detected. Pre-ANTU MLT administration led to significant decreases in PE, LW/BW, and PE/BW levels. The inhibited glutathione (GSH) and superoxide dismutase (SOD) levels and high malondialdehyde (MDA) levels that ANTU increase lipid peroxidation in the study. MLT administration eliminated oxidative stress by reducing MDA and ameliorating GSH and SOD levels. Pre-ANTU MLT administration led to a significant decrease in interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) levels in the lung when compared to the ANTU group without MLT administration. Post-pinealectomy ANTU administration significantly increased IL-1 beta and TNF-alpha levels when compared to ANTU and MLT administration without pinealectomy. Diffused inflammatory cell infiltration, interstitial pulmonary edema, and histopathological congestion were observed after the administration of ANTU. Severity of the damage was elevated in the ANTU + PINX group. MLT treatment regressed pulmonary effusion and edema and improves lung structure. In brief, the findings suggested that MLT inhibited proinflammatory mediators and could serve as a therapeutic agent to prevent inflammatory disorders.Öğe Protective effect of astaxanthin on testis torsion/detorsion injury through modulation of autophagy(Mre Press, 2024) Yilmaz, Nesibe; Yildiz, Azibe; Tanbek, Kevser; Kisaoglu, Aysegul; Yilmaz, Umit; Kose, EvrenA significant clinical condition known as testicular torsion leads to permanent ischemic damage to the testicular tissue and consequent loss of function in the testicles. In this study, it was aimed to evaluate the protective effects of Astaxanthin (ASTX) on testicular damage in rats with testicular torsion/detorsion in the light of biochemical and histopathological data. Spraque Dawley rats of 21 were randomly divided into three groups; sham, testicular torsion/detorsion (TTD) and astaxanthin + testicular torsion/detorsion (ASTX + TTD). TTD and ASTX + TTD groups underwent testicular torsion for 2 hours and then detorsion for 4 hours. Rats in the ASTX + TTD group were given 1 mg/kg/day astaxanthin by oral gavage for 7 days before torsion. Following the detorsion process, oxidative stress parameters and histopathological changes in testicular tissue were evaluated. Malondialdehyde (MDA) and total oxidant status (TOS) levels were significantly decreased in the ASTX group compared to the TTD group, while superoxide dismutase (SOD), glutathione (GSH) and total antioxidant status (TAS) levels were increased ( p < 0.05). Moreover, histopathological changes were significantly reduced in the group given ASTX ( p < 0.0001). It was determined that ASTX administration increased Beclin-1 immunoreactivity in ischemic testicular tissue, while decreasing caspase-3 immunoreactivity ( p < 0.0001). Our study is the first to investigate the antiautophagic and antiapoptotic properties of astaxanthin after testicular torsion/detorsion based on the close relationship of Beclin-1 and caspase-3 in ischemic tissues. Our results clearly demonstrate the protective effects of ASTX against ischemic damage in testicular tissue. In ischemic testicular tissue, ASTX contributes to the survival of cells by inducing autophagy and inhibiting the apoptosis.Öğe Sex and age estimation with corneal topography parameters by using machine learning algorithms and artificial neural networks(Int Assoc Law & Forensic Sciences, 2024) Yilmaz, Nesibe; Secgin, Yusuf; Mercan, KadirBackground The aim of this study, which was based on this hypothesis, was to estimate sex and age by using a machine learning algorithm (ML) and artificial neural networks (ANN) with parameters obtained from the eyeball. The study was conducted on corneal topography images of 155 women and 155 men aged between 6 and 87 who did not have surgical intervention or pathology in their eyeballs. In the study, the individuals were divided into four different age groups 6-17, 18-34, 35-55, and 56-87. Sex and age estimation was carried out by using the numerical data of parameters obtained as a result of corneal topography imaging in ML and ANN inputs.Results As a result of our study, in sex determination, a 0.98 accuracy rate (Acc) was obtained with the logistic regression algorithm, one of the ML algorithms, and 0.94 Acc was obtained with the MLCP model, one of the ANN algorithms; in age estimation, 0.84 Acc was obtained with RF algorithm, one of the ML algorithms. With the SHAP analyzer of the Random Forest algorithm, through which the effects of parameters on the overall result are evaluated, the parameter that made the highest contribution to sex estimation was found to be corneal volume, and the parameter that made the highest contribution to age estimation was found to be pupil Q parameter.Conclusion As a result of our study, it was found that parameters obtained from the eyeball showed a high accuracy in sex and age estimation.
