Disposable biogenic amine biosensors for histamine determination in fish
Küçük Resim Yok
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Royal Soc Chemistry
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This study presents the development of disposable biosensors employed in the determination of histamine in fish samples. Screen printed carbon electrodes (SPCEs) were first modified with a mixture of titanium dioxide nanoparticles (TiO2), carboxylated multiwalled carbon nanotubes (c-MWCNTs), hexaammineruthenium(iii) chloride (RU) and chitosan (CS). Diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes were further immobilized onto the TiO2-c-MWCNT-RU-CS/SPCEsvia1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) and hydroxysuccinimide (NHS) chemistry for the fabrication of the biosensors. The morphological and electrochemical properties of the proposed biosensors were studied using scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, cyclic voltammetry (CV), chronoamperometry and electrochemical impedance spectroscopy (EIS). A performance comparison of two biosensors indicated that the one based on DAO had a linear concentration range from 9.9 x 10(-6)to 1.1 x 10(-3)M and the other based on MAO, from 5.6 x 10(-5)to 1.1 x 10(-3)M for histamine. The sensitivity of the DAO based biosensor was almost 1.5 times higher than that of the MAO based biosensor. The proposed biosensors were successfully employed to determine histamine in fish samples and the recoveries were between 100.0% and 104.6%.
Açıklama
Anahtar Kelimeler
Carbon Nanotube, Amperometric Biosensor, Electrochemical Biosensor, Enzyme Sensor, Oxidase, Immobilization, Nanoparticles, Tyrosinase, Composite, Food
Kaynak
Analytical Methods
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
12
Sayı
30
