A Comparison of the Costs, Reliability and Time of Result Periods of Widely Used Methods, New Molecular Methods and MALDI Tof-Ms in the Routine Diagnosis of Candida Strains

Basit Öğe Kaydı
dc.contributor.authorCakmakliogullari, Elcin Kal
dc.contributor.authorAsgin, Nergis
dc.contributor.authorDegeri, Kenan
dc.date.accessioned2024-09-29T16:09:55Z
dc.date.available2024-09-29T16:09:55Z
dc.date.issued2019
dc.departmentKarabük Üniversitesien_US
dc.description.abstractIn recent years, the fast and accurate identification of the Candida species is of great importance as the response to antifungal treatment differs among species. Following the treatment of several immunosuppressive diseases, fungal infections can emerge. The aim of this study was to compare the accuracy, costs and time of result periods of the methods used in the identification of the most common human fungal infectious agent, Candida strains. From various clinical samples sent to the Microbiology Laboratory of Karabuk University Training and Research Hospital between July 2016-December 2017, a total of 91 yeast isolates cultivated in blood agar (Becton Dickinson, USA) and/or Sabouraud dextrose agar (SDA-Oxoid, UK), confirmed with colony morphology and microscopic appearance, identified as Candida species with a fully automated identification system (Phoenix (TM) Yeast ID Panel, Becton Dickinson Diagnostics, USA) were included in the study. All the samples were examined with sequence analysis using ITS1 forward 5'-TCC GTA GGT GAA CCT GCG G-3' and ITS4 reverse 5'-TCC TCC GCT TAT TGA TAT GC-3' primers (Iontek, Turkey) and the matrix-assisted laser desorption-ionisation time of flight mass spectrometry (MALDI TOF-MS) systems. Molecular sequence analysis was accepted as the gold standard method and the results were compared with those of the other methods MALDI TOF-MS and Phoenix (TM) Yeast ID Panel in respect of the accuracy of the identification of Candida strains. According to the results of the DNA sequence analysis of the 91 Candida isolates included in the study, 24 were identified as Candida albicans, 20 Candida tropicalis, 16 Candida parapsilosis, 13 Candida glabrata, seven Candida kefyr, six Candida krusei, two of each Candida dubliniensis, Candida guilliermondi and one Candida lusitaniae. Compared to the results of the DNA sequence analysis, the accurate identification of the fully automated Phoenix (TM) system and the MALDI TOF-MS system was found as 92.3% and 97.8%, respectively. In addition to accuracy, costs and time of result periods of the three methods were also compared. Disregarding the cost of the device in the 3 methods, when the comparison was made of the cost per test and the time to results after pure production in SDA agar, the MALDI TOF-MS system was determined to have the lowest costs and provided results in the shortest time. As some of the Candida strains have antifungal resistance, identification of the strains must be a priority in respect of starting early treatment. The MALDI TOF-MS system has high performance in accurate identification, low costs and the system provides the results within minutes, thereby allowing immediate decision to be made for the antifungal treatment to be started. Thus, the morbidity, mortality and cost rates will be reduced. In conclusion, as the MALDI TOF-MS is a rapid, reliable and low cost per test system, it can be considered suitable for routine use in laboratories.en_US
dc.identifier.doi10.5578/mb.67952
dc.identifier.endpage212en_US
dc.identifier.issn0374-9096
dc.identifier.issue2en_US
dc.identifier.pmid31130124en_US
dc.identifier.scopus2-s2.0-85066929189en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage204en_US
dc.identifier.trdizinid371576en_US
dc.identifier.urihttps://doi.org/10.5578/mb.67952
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/371576
dc.identifier.urihttps://hdl.handle.net/20.500.14619/7849
dc.identifier.volume53en_US
dc.identifier.wosWOS:000465377600008en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.publisherAnkara Microbiology Socen_US
dc.relation.ispartofMikrobiyoloji Bultenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCandidaen_US
dc.subjectstrain identificationen_US
dc.subjectMALDI TOF-MSen_US
dc.subjectmolecular sequence analysisen_US
dc.subjectPhoenix (TM) Yeast ID Panelen_US
dc.titleA Comparison of the Costs, Reliability and Time of Result Periods of Widely Used Methods, New Molecular Methods and MALDI Tof-Ms in the Routine Diagnosis of Candida Strainsen_US
dc.title.alternativeCandida Tür Tayininde Rutinde Yaygın Olarak Kullanılan Yöntemlerle Yeni Kullanılmaya Başlayan MALDI Tof-Ms ve Moleküler Yöntemlerin Sonuç Verme Süreleri, Maliyetleri ve Güvenilirliklerinin Karşılaştırılmasıen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu