Real-time PCR analysis of pyrethroid resistance in Helicoverpa armigera from Turkey

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dc.authoridKONUS, METIN/0000-0002-9953-1375
dc.contributor.authorKonus, Metin
dc.contributor.authorKaraagac, Sakine Ugurlu
dc.contributor.authorIscan, Mesude
dc.date.accessioned2024-09-29T16:09:50Z
dc.date.available2024-09-29T16:09:50Z
dc.date.issued2014
dc.departmentKarabük Üniversitesien_US
dc.description.abstractAim: Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) is a polyphagous pest of a wide range of crops such as cotton, tomato and soybean. Pyrethroid insecticides have commonly used against it in agricultural areas, but excess amount applications of them result in resistance development in the field populations of H. armigera. Resistance development usually occurs with increased metabolism of certain enzymatic systems such as CYP450, GST and esterases. Therefore, expressions of selected CYP450, GST and esterase genes of H. armigera field populations (Adana and Mardin) were compared to those of a susceptible strain by real-time PCR method for analyzing role of these systems in pyrethroid resistance development of H. armigera. Material and Methods: Real-Time PCR Method Results: It was found that H. armigera reacts to pyrethroids mainly by increasing expressions of CYP9A14 gene together with CYP4S1 and CYP9A12 genes. However, analyzed GST and esterase genes expression were not significantly changed in field populations. Conclusion: Consequently, while CYP450 enzyme system is actively involved in pyrethroid resistance, GSTs and esterases enzyme systems don't seem to be actively involved in resistance development against pyrethroid insecticides in H. armigera field populations from Turkey.en_US
dc.identifier.doi10.5505/tjb.2014.50490
dc.identifier.endpage180en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84903759051en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage176en_US
dc.identifier.urihttps://doi.org/10.5505/tjb.2014.50490
dc.identifier.urihttps://hdl.handle.net/20.500.14619/7807
dc.identifier.volume39en_US
dc.identifier.wosWOS:000338275000009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkish Biochem Socen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHelicoverpa armigeraen_US
dc.subjectresistanceen_US
dc.subjectqRT-PCRen_US
dc.subjectCYP450en_US
dc.subjectpyrethroid insecticidesen_US
dc.titleReal-time PCR analysis of pyrethroid resistance in Helicoverpa armigera from Turkeyen_US
dc.typeArticleen_US

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