Comparison of Mesenchymal Stem Cells Isolated From Pulp and Periodontal Ligament

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dc.authoridTurac, Gizem/0000-0001-8219-2015
dc.authoridDURUKSU, Gokhan/0000-0002-3830-2384
dc.authoridHAKKI, ERDOGAN ESREF/0000-0001-7147-7875
dc.authoridHakki, Sema/0000-0001-8665-6235
dc.authoridunal halbutogullari, zehra seda/0000-0001-6810-4939
dc.authoridKAYIS, Seyit Ali/0000-0003-4791-8946
dc.contributor.authorHakki, Sema S.
dc.contributor.authorKayis, Seyit Ali
dc.contributor.authorHakki, Erdogan E.
dc.contributor.authorBozkurt, S. Buket
dc.contributor.authorDuruksu, Gokhan
dc.contributor.authorUnal, Zehra Seda
dc.contributor.authorTurac, Gizem
dc.date.accessioned2024-09-29T16:06:39Z
dc.date.available2024-09-29T16:06:39Z
dc.date.issued2015
dc.departmentKarabük Üniversitesien_US
dc.description.abstractBackground: Cell-based therapy using mesenchymal stem cells (MSCs) seems promising to obtain regeneration of dental tissues. A comparison of tissue sources, including periodontal ligament (PDL) versus pulp (P), could provide critical information to select an appropriate MSC population for designing predictable regenerative therapies. The purpose of this study is to compare the proliferation and stemness and the MSC-specific and mineralized tissue-specific gene expression of P-MSCs and PDL-MSCs. Methods: MSCs were obtained from PDL and P tissue of premolars (n = 3) extracted for orthodontic reasons. MSC proliferation was evaluated using a real-time cell analyzer for 160 hours. Telomerase activity was evaluated by a telomeric repeat amplification protocol assay based on enzyme-linked immunosorbent assay. Total RNA was isolated from the MSCs on day 3. A polymerase chain reaction (PCR) array was used to compare the expression of MSC-specific genes. The expression of mineralized tissue-associated genes, including Type I collagen (COL I), runt-related transcription factor 2 (RunX2), bone sialoprotein (BSP), and osteocalcin (OCN) messenger RNA (mRNA), was evaluated using quantitative real-time PCR. Results: Higher proliferation potential and telomerase activity were observed in the P-MSCs compared to PDL-MSCs of premolar teeth. Fourteen of 84 genes related to MSCs were expressed differently in the PDL-MSCs versus the P-MSCs. The expressions of bone morphogenetic protein 2 (BMP2) and BMP6; sex-determining region Y-box 9 (SOX9); integrin, alpha 6 (ITGA6); melanoma cell adhesion molecule (MCAM); phosphatidylinositol glycan anchor biosynthesis, class S (PIGS); prominin 1 (PROM1); ribosomal protein L13A (RPL13A); and microphthalmia-associated transcription factor (MITF) were higher in the P-MSCs compared to the PDL-MSCs, and higher expression of matrix metalloproteinase 2 (MMP2), interleukin (IL)-6, insulin (INS), alanyl (membrane) aminopeptidase (ANPEP), and IL-10 were observed in the PDL-MSCs. However, there was no statistically significant difference in the expression of mineralized tissue-associated genes, including BSP and RunX2, between the P-MSCs and the PDL-MSCs. Higher expression of COL I and lower expression of OCN mRNA transcripts were noted in the PDL-MSCs compared to the P-MSCs. Conclusions: The results of this study suggest that MSCs isolated from P and PDL tissues show different cellular behavior. To increase the predictability of MSC-based regenerative treatment, differences in dental tissue-derived MSCs and favorable aspects of cell sources should be further clarified.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (Ankara, Turkey) [TUBITAK/SBAG-110S415]; Selcuk University Research Coordination Office, Konya, Turkey [BAP-10401104]en_US
dc.description.sponsorshipThis study was funded by the Scientific and Technological Research Council of Turkey (Ankara, Turkey) (TUBITAK/SBAG-110S415) (SSH) and Selcuk University Research Coordination Office, Konya, Turkey (BAP-10401104) (SSH). This work was performed at Research Center of Dental Faculty, Selcuk University, Konya, Turkey, and Kocaeli University, Center for Stem Cell and Gene Therapies, Research, and Practice, Institute of Health Sciences, Stem Cell Department, Kocaeli, Turkey. The authors report no conflicts of interest related to this study.en_US
dc.identifier.doi10.1902/jop.2014.140257
dc.identifier.endpage291en_US
dc.identifier.issn0022-3492
dc.identifier.issn1943-3670
dc.identifier.issue2en_US
dc.identifier.pmid25325708en_US
dc.identifier.scopus2-s2.0-84924943894en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage283en_US
dc.identifier.urihttps://doi.org/10.1902/jop.2014.140257
dc.identifier.urihttps://hdl.handle.net/20.500.14619/6967
dc.identifier.volume86en_US
dc.identifier.wosWOS:000349380500014en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Periodontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell biologyen_US
dc.subjectdifferentiationen_US
dc.subjectgene expressionen_US
dc.subjectmesenchymal stem cellen_US
dc.subjectmolecular biologyen_US
dc.subjectregenerative medicineen_US
dc.titleComparison of Mesenchymal Stem Cells Isolated From Pulp and Periodontal Ligamenten_US
dc.typeArticleen_US

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