Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

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dc.authoridDalgic, Ali Deniz/0000-0003-2904-1204
dc.authoridTezcaner, Aysen/0000-0003-4292-5856
dc.authoridEvis, Zafer/0000-0002-7518-8162
dc.authoridAlshemary, Ammar Z/0000-0001-5367-1869
dc.contributor.authorDalgic, Ali Deniz
dc.contributor.authorAlshemary, Ammar Z.
dc.contributor.authorTezcaner, Aysen
dc.contributor.authorKeskin, Dilek
dc.contributor.authorEvis, Zafer
dc.date.accessioned2024-09-29T16:05:00Z
dc.date.available2024-09-29T16:05:00Z
dc.date.issued2018
dc.departmentKarabük Üniversitesien_US
dc.description.abstractIn this study, novel graphene oxide-incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(epsilon-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(epsilon-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(epsilon-caprolactone) - 10% silicate-doped nano-hydroxyapatite - 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.en_US
dc.identifier.doi10.1177/0885328218763665
dc.identifier.endpage1405en_US
dc.identifier.issn0885-3282
dc.identifier.issn1530-8022
dc.identifier.issue10en_US
dc.identifier.pmid29544381en_US
dc.identifier.scopus2-s2.0-85044019896en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1392en_US
dc.identifier.urihttps://doi.org/10.1177/0885328218763665
dc.identifier.urihttps://hdl.handle.net/20.500.14619/6440
dc.identifier.volume32en_US
dc.identifier.wosWOS:000432082400007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofJournal of Biomaterials Applicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGraphene oxideen_US
dc.subjectsilicate-doped hydroxyapatiteen_US
dc.subjectpoly(epsilon-caprolactone)en_US
dc.subjectwet-electrospinningen_US
dc.subjectbone tissue engineeringen_US
dc.titleSilicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineeringen_US
dc.typeArticleen_US

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