Identification of bisecting N-glycans in tandem mass spectra using a procainamide labeling approach for in-depth N-glycan profiling of biological samples
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Bisecting N-glycan structures, which are commonly observed in glycoproteins, regulate many important functions in organisms. Tandem mass spectrometry is the most frequently utilized approach for the identification of bisecting N-glycan structures. However, it can be difficult to obtain fragments to recognize bisecting N-glycans based on the analysis performed, i.e., by using hydrophilic interaction liquid chromatography equipped with a fluorescence detector and a quadrupole time-of-flight tandem mass spectrometry (HILIC-FLD-QTOF-MS/MS). The misinterpretation of bisecting N-glycans for other types of N-glycans is possible. Therefore, a method is needed to specifically recognize bisecting N-glycan structures. This report introduces a facile strategy based on tandem mass spectrometry to identify bisecting N-glycans by using a procainamide labeling approach that increases both the mass spectrometric and the fluorescence detection sensitivity of the N-glycans. In this strategy, the precursor ions belonging to bisecting N-glycans were used by extracting the detected diagnostic fragment ions, including proc-H1N3 (m/z 1009.481+) and proc-H1N3F1 (m/z 1155.5391, in the corresponding tandem mass spectra. Subsequently, the structures of the bisecting N-glycans were confirmed. The presented strategy was applied to human IgG glycoprotein and human plasma glycoproteome. Finally, stepping energy-collision induced dissociation (SE-CID) was applied to validate the diagnostic fragments. This approach enables bisecting N-glycans to be verified and can be used for further mass spectrometry-based glycan analysis of biological samples. (C) 2020 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Bisecting N-Glycans, Tandem mass spectrometry, Procainamide labeling, Glycosylation
Kaynak
International Journal of Mass Spectrometry
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
457
