Spexin may induce mitochondrial biogenesis in white and brown adipocytes via the hypothalamus-pituitary-thyroid (HPT) axis

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dc.authoridYilmaz, Umit/0000-0003-0248-3483
dc.authoridTanbek, Kevser/0000-0003-2099-2273
dc.contributor.authorYilmaz, Umit
dc.contributor.authorTanbek, Kevser
dc.date.accessioned2024-09-29T16:00:32Z
dc.date.available2024-09-29T16:00:32Z
dc.date.issued2024
dc.departmentKarabük Üniversitesien_US
dc.description.abstractAim: The present study aimed to investigate the effect of the intracerebroventricular (icv) administration of spexin on the hypothalamus-pituitary-thyroid (HPT) axis (TRH, TSH, T4 and T3 hormones) and energy expenditure (PGC-1 alpha and UCP1 genes) in white adipose (WAT) and brown adipose tissues (BAT) in rats. Furthermore, the study aimed to determine the effects of spexin on food-water consumption and body weight of rats.Material and method: The study was conducted with 40 male rats that were divided into 4 groups: Control, Sham, Spexin 30 and Spexin 100 (n = 10). Spexin (1 mu l/hour) was administered to rats other than those in the control group for 7 days with osmotic minipumps intracerebroventricularly, artificial cerebrospinal fluid (vehicle) was administered to the Sham group, and 30 nMol and 100 nMol spexin was infused to the Spexin 30 and Spexin 100 groups, respectively. Food-water consumption and body weight of the rats were monitored during the experiments. After the seven-day infusion, the rats were decapitated and serum TSH, fT4 and fT3 levels were determined with ELISA on rat blood samples. Also, TRH gene expression levels from the hypothalamus tissues and PGC-1 alpha and UCP1 expression levels from WAT and BAT were determined by real-time PCR.Findings: It was determined that icv spexin infusion reduced daily food consumption and body weight without leading to a significant change in water consumption (p < 0.05). Icv spexin infusion significantly decreased serum TSH, and increased fT4 and fT3 levels when compared to control and sham groups (p < 0.05). Moreover, icv spexin infusion increased the TRH expressions in the hypothalamus tissues and PGC-1 alpha UCP1 in the WAT and BAT (p < 0.05).Conclusion: Icv Spexin infusion may have effects on food consumption and body weight as well as, thyroid hormones and energy metabolism.en_US
dc.description.sponsorshipResearch Fund of Karabuk University [22 -ABP -098]; Karabuk University, Experimental Animal Ethics Committee [22/6/9]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Karabuk University [Project No: 22-ABP-098]. This study was approved by the Karabuk University, Experimental Animal Ethics Committee (Protocol No: 22/6/9).en_US
dc.identifier.doi10.1016/j.physbeh.2023.114401
dc.identifier.issn0031-9384
dc.identifier.issn1873-507X
dc.identifier.pmid37939828en_US
dc.identifier.scopus2-s2.0-85176120540en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.physbeh.2023.114401
dc.identifier.urihttps://hdl.handle.net/20.500.14619/5208
dc.identifier.volume273en_US
dc.identifier.wosWOS:001111628400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofPhysiology & Behavioren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIntracerebroventricular (icv)en_US
dc.subjectSpexinen_US
dc.subjectThyroiden_US
dc.subjectEnergy expenditureen_US
dc.subjectMitochondrial biogenesisen_US
dc.subjectAdipocyteen_US
dc.titleSpexin may induce mitochondrial biogenesis in white and brown adipocytes via the hypothalamus-pituitary-thyroid (HPT) axisen_US
dc.typeArticleen_US

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