Expression of hypoxia-inducible factors and vascular endothelial growth factor during pregnancy in the feline uterus

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dc.authoridOzmen, Ozlem/0000-0002-1835-1082
dc.authoridKAYIS, Seyit Ali/0000-0003-4791-8946
dc.authoridAgaoglu, Ali Reha/0000-0002-6545-8800
dc.contributor.authorAgaoglu, Ozgecan Korkmaz
dc.contributor.authorAgaoglu, Ali Reha
dc.contributor.authorGuzeloglu, Aydin
dc.contributor.authorKurar, Ercan
dc.contributor.authorKayis, Seyit Ali
dc.contributor.authorOzmen, Ozlem
dc.contributor.authorSchafer-Somi, Sabine
dc.date.accessioned2024-09-29T16:00:48Z
dc.date.available2024-09-29T16:00:48Z
dc.date.issued2015
dc.departmentKarabük Üniversitesien_US
dc.description.abstractHypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HIF gene family and VEGF in the cat uterus during pregnancy. Tissue samples of the whole uterine wall were collected after ovariohysterectomy and allocated to the following groups: embryo positive (group 1 [G1], n = 7, 7 days after mating), early pregnancy (group 2 [G2], n = 7, 20 days after mating), mid-pregnancy (group 3 [G3], n = 7, 24 days after mating), late pregnancy (group 4 [G4], n = 7,30-45 days after mating), and oocyte positive groups (group 5 [G5], n = 7, 7 days after induction of ovulation with GnRH analog). Relative mRNA levels were determined by real-time polymerase chain reaction. As housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase was used. The relative gene expression of HIF1A in G5 was found to be significantly higher than that of other groups (G1, G2, G3, and G4) (P < 0.05). In addition, the expression of HIF2A in G5 was higher than that of Cl and HIF2A gene expression at placentation sites of G4 was higher than in G1, G2, and G3 (P < 0.05). Immunohistochemistry indicated that HIF1A, HIF2A, and VEGF expressions were observed in different cell types of uterine and placental tissues in late pregnancy and oocyte groups. The expression of HIF3A did not change significantly in any group investigated. These observations suggest that HIFs and VEGF may play a role in the establishment and development of pregnancy. (C) 2015 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112O878]en_US
dc.description.sponsorshipThis study was financed by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No. 112O878 and the project coordinator was Dr. OKA).en_US
dc.identifier.doi10.1016/j.theriogenology.2015.02.009
dc.identifier.endpage33en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue1en_US
dc.identifier.pmid25794839en_US
dc.identifier.scopus2-s2.0-84929943005en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage24en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2015.02.009
dc.identifier.urihttps://hdl.handle.net/20.500.14619/5374
dc.identifier.volume84en_US
dc.identifier.wosWOS:000355572900004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHypoxia-inducible factoren_US
dc.subjectFelineen_US
dc.subjectUterusen_US
dc.subjectReal-time PCRen_US
dc.subjectVascular endothelial growth factoren_US
dc.titleExpression of hypoxia-inducible factors and vascular endothelial growth factor during pregnancy in the feline uterusen_US
dc.typeArticleen_US

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