Fast purification of glycans and glycopeptides using silk-packed micropipette tip for matrix-assisted laser desorption/ionization-mass spectrometry and high-performance liquid chromatography-fluorescence detection analysis

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dc.authoridBarlas, Nazire/0000-0002-6671-6090
dc.authoridATAKAY, MEHMET/0000-0002-7102-2974
dc.contributor.authorKayili, Haci Mehmet
dc.contributor.authorBarlas, Nazire
dc.contributor.authorAtakay, Mehmet
dc.contributor.authorSalih, Bekir
dc.date.accessioned2024-09-29T15:57:57Z
dc.date.available2024-09-29T15:57:57Z
dc.date.issued2018
dc.departmentKarabük Üniversitesien_US
dc.description.abstractA novel micropipette tip packed with a natural product generated from silk worms is introduced in this study for the specific separation and purification of glycopeptides and glycans. The enrichment and purification performances of the silk-packed micropipette tip were investigated for both glycopeptide and glycan levels. Site specific glycosylation analysis of tryptic IgG Fc glycopeptides by MALDI-MS was achieved using a protocol containing enrichment step with the silk-packed micropipette tip. The enrichment efficiency and specificity of the silk-packed pipette tip regarding to glycopeptides and glycans was evaluated using the digests of some glycoproteins having neutral and sialylated glycopeptides and glycans. The MS and HPLC-FLD results indicate that the silk packed micropipette tip has good adsorption capacity to glycoconjugates in HILIC enrichment mode. In addition, purification of 2-aminobenzoic acid labelled IgG, fetuin and human plasma N-glycans with silk-packed micropipette tip was performed, and thus salts (PBS), detergents (SDS, NP-40) and interfered solvents (such as DMSO etc.) were removed prior to MS and HPLC-FLD analyses. (C) 2018 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [115Z241]; Hacettepe University The Scientific Research Projects Coordination Unit [FAY-2015-7201]; Turkish Academy of Sciences (TUBA)en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) with the project Number: 115Z241. We thank to Hacettepe University The Scientific Research Projects Coordination Unit for the partial financial support with the Project Number: FAY-2015-7201. B. Salih gratefully acknowledges the Turkish Academy of Sciences (TUBA) for the partial financial support. We thank to Dr. Cengiz Uzun for providing raw silk products. Authors have no conflict of interest related to manuscript.en_US
dc.identifier.doi10.1016/j.microc.2018.03.034
dc.identifier.endpage499en_US
dc.identifier.issn0026-265X
dc.identifier.issn1095-9149
dc.identifier.scopus2-s2.0-85044856604en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage492en_US
dc.identifier.urihttps://doi.org/10.1016/j.microc.2018.03.034
dc.identifier.urihttps://hdl.handle.net/20.500.14619/5113
dc.identifier.volume139en_US
dc.identifier.wosWOS:000433268700063en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofMicrochemical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlycopeptide enrichmenten_US
dc.subjectGlycan purificationen_US
dc.subjectHILICen_US
dc.subjectMass spectrometryen_US
dc.subjectSilken_US
dc.titleFast purification of glycans and glycopeptides using silk-packed micropipette tip for matrix-assisted laser desorption/ionization-mass spectrometry and high-performance liquid chromatography-fluorescence detection analysisen_US
dc.typeArticleen_US

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