Intracerebroventricular PROK2 infusion could increase the secretion of male reproductive hormones by stimulating the HPG axis

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dc.authoridyilmaz, nesibe/0000-0002-5527-8507
dc.contributor.authorYilmaz, Nesibe
dc.contributor.authorYildiz, Azibe
dc.date.accessioned2024-09-29T15:51:23Z
dc.date.available2024-09-29T15:51:23Z
dc.date.issued2024
dc.departmentKarabük Üniversitesien_US
dc.description.abstractBackgroundProkineticin 2 (PROK2), an important neuropeptide that plays a key role in the neuronal migration of gonadotropin-releasing hormone (GnRH) in the hypothalamus, is known to have regulatory effects on the gonads. In the present study, the impact of intracerebroventricular (icv) PROK2 infusion on hypothalamic-pituitary-gonadal axis (HPG) hormones, testicular tissues, and sperm concentration was investigated.Methods and resultsRats were randomly divided into four groups: control, sham, PROK2 1.5 and PROK2 4.5. Rats in the PROK2 1.5 and PROK2 4.5 groups were administered 1.5 nmol and 4.5 nmol PROK2 intracerebroventricularly for 7 days via an osmotic mini pump (1 mu l/h), respectively. Rat blood serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone hormone levels were determined with the ELISA method in the blood samples after 7 days of infusion. GnRH mRNA expression was determined with the RT-PCR in hypothalamus tissues. analyze Sperm concentration was determined, and testicular tissue was examined histologically with the hematoxylin-eosin staining method. It was observed that GnRH mRNA expression increased in both PROK2 infusion groups. Serum FSH, LH and testosterone hormone levels also increased in these groups. Although sperm concentration increased in PROK2 infusion groups when compared to the control and sham, the differences were not statistically significant. Testicular tissue seminiferous epithelial thickness was higher in the PROK2 groups when compared to the control and sham groups.ConclusionThe present study findings demonstrated that icv PROK2 infusion induced the HPG axis. It could be suggested that PROK2 could be a potential agent in the treatment of male infertility induced by endocrinological defects.en_US
dc.description.sponsorshipKarabuk University Scientific Research Projects Department [KBUBAP-22-ABP-099]en_US
dc.description.sponsorshipThe present study (Project no: KBUBAP-22-ABP-099) was sponsored by the Karabuk University Scientific Research Projects Departmenten_US
dc.identifier.doi10.1007/s11033-024-09604-4
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1en_US
dc.identifier.pmid38740671en_US
dc.identifier.scopus2-s2.0-85192797374en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-024-09604-4
dc.identifier.urihttps://hdl.handle.net/20.500.14619/4029
dc.identifier.volume51en_US
dc.identifier.wosWOS:001221717600001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPROK2en_US
dc.subjectGnRHen_US
dc.subjectFSHen_US
dc.subjectLHen_US
dc.subjectTestosteronen_US
dc.subjectSperm concentrationen_US
dc.titleIntracerebroventricular PROK2 infusion could increase the secretion of male reproductive hormones by stimulating the HPG axisen_US
dc.typeArticleen_US

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