Antibiotic resistance and molecular epidemiology of vancomycin-resistant enterococci in a tertiary care hospital in Turkey

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dc.contributor.authorAsgin, N.
dc.contributor.authorOtlu, B.
dc.date.accessioned2024-09-29T16:16:26Z
dc.date.available2024-09-29T16:16:26Z
dc.date.issued2020
dc.departmentKarabük Üniversitesien_US
dc.description.abstractPurpose: Vancomycin-resistant enterococci (VRE) have become a global health threat in the last two decades. In this study, we aimed to determine antibiotic resistance using phenotypic and genotypic methods in VRE strains obtained from inpatients and to investigate clonal relatedness among strains. Methods: Identification and antibiotic susceptibility of 47 VRE strains obtained from inpatients at Karabuk University Hospital from 2014 to 2015 were determined using the BD Phoenix™ automated microbiology system. Vancomycin resistance genes (Van A and B) were detected by polymerase chain reaction. Clonal relatedness among the strains was evaluated by pulsed-field gel electrophoresis (PFGE). Results: All 47 VRE strains obtained from rectal (n=35), blood (n=7), and urine (n=5) samples were confirmed as Enterococcus faecium; they were resistant to ampicillin, genta-micin, vancomycin, and teicoplanin. One E. faecium isolate was intermediately resistant to linezolid. No strain was resistant to quinupristin–dalfopristin or daptomycin. Only vanA was detected among strains. According to the PFGE results, 31 of 47 strains were clonally related with a clustering rate of 66%. No common clone was detected. Conclusion: VRE infections are associated with high mortality, morbidity, and healthcare expenditures. Increasing resistance to last-line drugs, such as linezolid and daptomycin, among VRE strains is a great concern. Therefore, comprehensive measures should be performed to reduce VRE colonization. Although there was no common clone VRE outbreak, polyclonal spread was observed in our hospital. The high clustering rate indicated cross-contamination. Thus, a more effective infection control program should be implemented. © 2020 Asgin and Otlu.en_US
dc.description.sponsorshipKarabuk University Scientific Research Project Unit, (KBU-BAP-KT-061); Ahi Evran Üniversitesi, AEUen_US
dc.identifier.doi10.2147/IDR.S191881
dc.identifier.endpage198en_US
dc.identifier.issn1178-6973
dc.identifier.scopus2-s2.0-85078799472en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage191en_US
dc.identifier.urihttps://doi.org/10.2147/IDR.S191881
dc.identifier.urihttps://hdl.handle.net/20.500.14619/9069
dc.identifier.volume13en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherDove Medical Press Ltd.en_US
dc.relation.ispartofInfection and Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectEnterococcus faeciumen_US
dc.subjectGastrointestinal tracten_US
dc.subjectLinezolid resistanceen_US
dc.subjectPulsed-field gel electrophoresisen_US
dc.titleAntibiotic resistance and molecular epidemiology of vancomycin-resistant enterococci in a tertiary care hospital in Turkeyen_US
dc.typeArticleen_US

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