Evaluation of the Association Between CRNDE Plasma Expression Level, KRAS, NRAS, and BRAF Variants in Patients with advanced CRC

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dc.authoridArslan, SERAP/0000-0002-7112-5658
dc.contributor.authorArslan, Serap
dc.contributor.authorDincer, Murat
dc.contributor.authorGarbioglu, Duygu B. A. Y. I. R.
dc.contributor.authorOzen, Hulya
dc.contributor.authorAras, Beyhan D. U. R. A. K.
dc.contributor.authorArtan, Sevilhan
dc.contributor.authorCilingir, Oguz
dc.date.accessioned2024-09-29T16:09:36Z
dc.date.available2024-09-29T16:09:36Z
dc.date.issued2023
dc.departmentKarabük Üniversitesien_US
dc.description.abstractColorectal cancer is one of the most common types of cancer. CRNDE is a novel-defined lncRNA. We evaluated the possibility of using CRDNE circulating lncRNA as a noninvasive biomarker. Also, we examined the effect of circulating CRNDE lncRNA on the pathogenesis of CRC and its association with the KRAS, NRAS, and BRAF somatic variants commonly observed in CRC. In this study, we enrolled plasma and FFPE tissue samples of 50 advanced CRC patients and plasma samples of 31 individuals in the control group of similar ages. Then, we performed plasma extraction, total RNA isolation, cDNA synthesis, and circulating lncRNA expression analysis, respectively. Also, we carried out KRAS, NRAS, and BRAF somatic variant analysis from FFPE tissues. Our results showed that the expression level of CRNDE (p= 0.002) were significantly upregulated in the CRC when it was compared to with the control group. The calculated area under the curve of the receiver operating characteristic was 0.70. We found a statistically significant difference between the KRAS somatic variants and the circulating CRNDE lncRNA (p= 0.031). This study demonstrated that CRNDE circulating lncRNAs may be used as a potential non-invasive biomarker in CRC. In our study, it was determined that there is a significant relationship between the frequently observed KRAS somatic variants and CRNDE in advanced-stage CRC cases. To the best of our knowledge, this is the first study to show the association of CRNDE circulating lncRNA with advanced CRC. Moreover, it is the first study to show a relationship between the KRAS somatic variants between circulating CRNDE lncRNA in advanced CRC.en_US
dc.description.sponsorshipResearch Foundation of Eskisehir Os-mangazi University [202011036]en_US
dc.description.sponsorshipAcknowledgements : This study was supported by the Research Foundation of Eskisehir Os-mangazi University. (June 12, 2020, Project No: 202011036) . .en_US
dc.identifier.doi10.4999/uhod.236797
dc.identifier.endpage151en_US
dc.identifier.issn1306-133X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85175644140en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage144en_US
dc.identifier.urihttps://doi.org/10.4999/uhod.236797
dc.identifier.urihttps://hdl.handle.net/20.500.14619/7666
dc.identifier.volume33en_US
dc.identifier.wosWOS:001085924200004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAkad Doktorlar Yayinevien_US
dc.relation.ispartofUhod-Uluslararasi Hematoloji-Onkoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCRNDEen_US
dc.subjectCirculating lncRNAen_US
dc.subjectKRASen_US
dc.subjectCRCen_US
dc.subjectRT-PCRen_US
dc.titleEvaluation of the Association Between CRNDE Plasma Expression Level, KRAS, NRAS, and BRAF Variants in Patients with advanced CRCen_US
dc.typeArticleen_US

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