Cellulose acetate-gelatin-coated boron-bioactive glass biocomposite scaffolds for bone tissue engineering

Basit Öğe Kaydı
dc.authoridAlshemary, Ammar Z/0000-0001-5367-1869
dc.authoridEvis, Zafer/0000-0002-7518-8162
dc.authoridTezcaner, Aysen/0000-0003-4292-5856
dc.authorid/0000-0001-7824-488X
dc.contributor.authorRad, Reza Moonesi
dc.contributor.authorAlshemary, Ammar Z.
dc.contributor.authorEvis, Zafer
dc.contributor.authorKeskin, Dilek
dc.contributor.authorTezcaner, Aysen
dc.date.accessioned2024-09-29T16:03:03Z
dc.date.available2024-09-29T16:03:03Z
dc.date.issued2020
dc.departmentKarabük Üniversitesien_US
dc.description.abstractIn this study, we aimed to prepare and characterize porous scaffolds composed of pure and boron oxide (B2O3)-doped bioactive glass (BG) that were infiltrated by cellulose acetate-gelatin (CA-GE) polymer solution for bone tissue engineering applications. Composite scaffolds were cross-linked with glutaraldehyde after polymer coating to protect the structural integrity of the polymeric-coated scaffolds. The impact of B(2)O(3)incorporation into BG-polymer porous scaffolds on the cross-sectional morphology, porosity, mechanical properties, degradation and bioactivity of the scaffolds was investigated. Human dental pulp stem cells (hDPSCs) were enzymatically isolated and used for cell culture studies. According to scanning electron microscope analysis, the porous structure of the scaffolds was preserved after polymer coating. After polymer infiltration, the porosity of the scaffolds decreased from 64.2% to 59.35% for pure BG scaffolds and from 67.3% to 58.9% for B2O3-doped scaffolds. Meanwhile, their compressive strengths increased from 0.13 to 0.57 MPa and from 0.20 to 0.82 MPa, respectively. After polymer infiltration, 7% B2O3-incorporated BG scaffolds had higher weight loss and Ca-P layer deposition than pure BG scaffolds, after 14 d of incubation in simulated body fluid at 37 degrees C. Higher attachment and proliferation of hDPSCs were observed on 7% B2O3-BG-CA/GE scaffolds. In addition, the alkaline phosphatase activity of the cells was about 1.25-fold higher in this group than that observed on BG-CA/GE scaffolds after 14 d of incubation in osteogenic medium, while their intracellular calcium amounts were 1.7-fold higher than observed on BG-CA/GE after 7 d of incubation in osteogenic medium. Our results suggested that porous cellulose acetate-gelatin-coated boron-BG scaffolds hold promise for bone tissue engineering applications.en_US
dc.description.sponsorshipBIOMATEN in METUen_US
dc.description.sponsorshipWe thank BIOMATEN in METU for their support of this research.en_US
dc.identifier.doi10.1088/1748-605X/ab8d47
dc.identifier.issn1748-6041
dc.identifier.issn1748-605X
dc.identifier.issue6en_US
dc.identifier.pmid32340000en_US
dc.identifier.scopus2-s2.0-85092475898en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1088/1748-605X/ab8d47
dc.identifier.urihttps://hdl.handle.net/20.500.14619/5865
dc.identifier.volume15en_US
dc.identifier.wosWOS:000575548300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherIop Publishing Ltden_US
dc.relation.ispartofBiomedical Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbone tissue engineeringen_US
dc.subjectnano bioactive glassen_US
dc.subjectboronen_US
dc.subjectnatural polymersen_US
dc.subjecthuman dental pulp stem cellsen_US
dc.subjectosteogenesisen_US
dc.titleCellulose acetate-gelatin-coated boron-bioactive glass biocomposite scaffolds for bone tissue engineeringen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu