Development of Amperometric Biosensor for Phenolic Compounds Using a Modified Electrode with Poly(GMA-co-MTM) and Laccase

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dc.authoridCelik-Kucuk, Asuman/0000-0002-3509-1497
dc.contributor.authorSulak, Meral Topcu
dc.contributor.authorErhan, Elif
dc.contributor.authorKeskinler, Bulent
dc.contributor.authorYilmaz, Faruk
dc.contributor.authorCelik, Asuman
dc.date.accessioned2024-09-29T16:04:54Z
dc.date.available2024-09-29T16:04:54Z
dc.date.issued2010
dc.departmentKarabük Üniversitesien_US
dc.description.abstractA new polymeric matrix, the copolymer of 3-thienylmethyl methacrylate (MTM) and glycidyl methacrylate (GMA), was used to develop a phenol biosensor based on laccase (TvLac). Chemical immobilization of the enzyme, TvLac, was achieved via the epoxy moiety of GMA onto the GMA-co-MTM coated glassy carbon electrode. GC/(GMA-co-MTM)/TvLac working electrode was investigated for the parameters of linear range, sensitivity, stability, pH and detection limit. Amperometric response was measured as a function of concentration of phenolic compounds, at a fixed bias voltage of -200 mV. The sensitivity in the linear range increased in this order with the GC/(GMA-co-MTM)/TvLac: 3-Chlorophenol > 2-Chlorophenol > 2,6-Dimethoxyphenol > Catechol > 4Methoxyphenol > 4-Chlorophenol > Phenol > Hydroquinone > 2,4-Dichlorophenol > p-Benzoquinone. The biosensor exhibited good sensitivity, stability for the tested phenolics compared to the reported working electrodes. It retained 65% of its initial activity after using for 300 measurements in one month.en_US
dc.description.sponsorshipGYTE Research Funden_US
dc.description.sponsorshipThe authors are grateful to GYTE Research Fund for its financial support of this work.en_US
dc.identifier.doi10.1166/sl.2010.1260
dc.identifier.endpage267en_US
dc.identifier.issn1546-198X
dc.identifier.issn1546-1971
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-77954900293en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage262en_US
dc.identifier.urihttps://doi.org/10.1166/sl.2010.1260
dc.identifier.urihttps://hdl.handle.net/20.500.14619/6399
dc.identifier.volume8en_US
dc.identifier.wosWOS:000276808500009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAmer Scientific Publishersen_US
dc.relation.ispartofSensor Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLaccaseen_US
dc.subjectPhenolen_US
dc.subjectAmperometric Biosensoren_US
dc.subjectEnzyme Electrodesen_US
dc.subjectPolymeric Matrixen_US
dc.titleDevelopment of Amperometric Biosensor for Phenolic Compounds Using a Modified Electrode with Poly(GMA-co-MTM) and Laccaseen_US
dc.typeArticleen_US

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