Determination of organophosphate resistance status and mechanism in Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) from Turkey

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dc.authoridKONUS, METIN/0000-0002-9953-1375
dc.contributor.authorKaraagac, Sakine Ugurlu
dc.contributor.authorKonus, Metin
dc.date.accessioned2024-09-29T16:06:17Z
dc.date.available2024-09-29T16:06:17Z
dc.date.issued2015
dc.departmentKarabük Üniversitesien_US
dc.description.abstractObjective: The objectives of this study were to determine resistance status to malathion and pirimiphos-methyl insecticides and to make biochemical analysis of resistance mechanism(s) developed to these insecticides in Sitophilus zeamais (S. zeamais) populations, collected from two different locations in Turkey. Two organophosphate insecticides, malathion and pirimiphos-methyl, were examined by bioassay using a discriminating dosage technique with impregnated filter papers. Mortality percentages were determined at the discriminating doses of these insecticides. In addition, esterase, glutathione S-transferase, and cytochrome P450 monooxygenase activities were also determined in this study in order to analyze detoxification mechanism(s) of tested insecticides in S. zeamais. Methods: Bioassay experiments of malathion and pirimiphos-methyl insecticides in S. zeamais populations were performed according to the IRAC susceptibility test method No:006. Furthermore, enzyme activities of esterases, cytochrome P450 monooxygenases, and glutathione S-transferases were determined by using biochemical assays. Results: The bioassay results of malathion and pirimiphos-methyl showed that only Kirikkale population of S. zeamais has resistance to both malathion and pirimiphos-methyl insecticides. However, no resistance was detected to malathion and pirimiphos-methyl insecticides in Samsun population of S. zeamais. Additionally, biochemical analysis displayed that while CYP450-PNOD activities showed an increase only in Kirikkale population (3.0-fold), EST-PNPA activities showed an increase only in Samsun population (1.3-fold). Finally, GST-CDNB activities increased both in Kirikkale (1.4-fold) and Samsun (2.2-fold) populations of S. zeamais compared to susceptible population. Conclusion: Consequently, cytochrome P450 monooxygenases and glutathione S-transferases seem to play a role in organophosphate resistance in Kirikkale population of S. zeamais from Turkey.en_US
dc.description.sponsorshipYuzuncu Yil University (BAP Project) [2013-FEN-B058]en_US
dc.description.sponsorshipThis study was supported by Yuzuncu Yil University (BAP Project No. 2013-FEN-B058).en_US
dc.identifier.doi10.1515/tjb-2015-0033
dc.identifier.endpage422en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84943543659en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage417en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2015-0033
dc.identifier.urihttps://hdl.handle.net/20.500.14619/6732
dc.identifier.volume40en_US
dc.identifier.wosWOS:000362432300010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSitophilus zeamaisen_US
dc.subjectinsecticide resistanceen_US
dc.subjectbiochemical mechanismsen_US
dc.subjectmalathionen_US
dc.subjectpirimiphos-methylen_US
dc.titleDetermination of organophosphate resistance status and mechanism in Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) from Turkeyen_US
dc.typeArticleen_US

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