Investigation of the Effect of Astaxanthin on Autophagy in Renal Ischemia-reper fusion Modeled Rats

Basit Öğe Kaydı
dc.contributor.authorKisaoglu, Aysegul
dc.contributor.authorKose, Evren
dc.contributor.authorYilmaz, Nesibe
dc.contributor.authorTanbek, Kevser
dc.contributor.authorYildiz, Azibe
dc.contributor.authorYilmaz, Umit
dc.contributor.authorCirik, Rumeyza Hilal
dc.date.accessioned2024-09-29T16:08:32Z
dc.date.available2024-09-29T16:08:32Z
dc.date.issued2024
dc.departmentKarabük Üniversitesien_US
dc.description.abstractObjective: The aim of this study was to investigate the effect of various astaxanthin (ATX) doses on oxidative damage and autophagy in renal ischemia-reperfusion (I/R) injury-modeled rats. Methods: The rats were divided into five groups: sham group (n=8), I/R (n=8), I/R + 5 mg/kg ATX (n=8), I/R + 10 mg/kg ATX (n=8), and I/R + 25 mg/ kg ATX (n=8) groups. ATX was dissolved in 5 mg/kg, 10 mg/kg, and 25 mg/ kg olive oil for 7 days and administered to the rats in the experimental group. Sham and I/R groups were also administered ATX solution (olive oil) via oral gavage for 7 days. Renal ischemia reperfusion was induced in all rats except the sham group after the last dose was administered on the 7 th day. Reperfusion was conducted for 24 hours after 45 minutes of ischemia. Results: Blood samples were collected, and kidney tissue were incised for biochemical and histological analyses. Superoxide dismutase (SOD) and total antioxidant status (TAS) were significantly lower in the I/R group than in the sham group (p<0.05), whereas malondialdehyde (MDA) and total oxidant status (TOS) values were higher (p<0.05). It was determined that SOD and TAS increased and MDA and TOS decreased in the ATXadministration groups compared with the I/R group, independent of the dose (p<0.05). In the 25 mg/kg ATX + I/R group, Beclin-1 and LC3 0 immunoreactivities were significantly higher than those in the other groups (p<0.05). The lowest p62 immunoreactivity was observed in the 25 mg/kg ATX + I/R group. Conclusions: ATX had a protective effect on kidney function and against oxidative damage. Furthermore, high-dose ATX administration protected kidney tissue via autophagy induction in this study.en_US
dc.description.sponsorshipResearch Fund of Inonu University [TYL-2020-2009]en_US
dc.description.sponsorshipThis study was supported by the Research Fund of Inonu University, Project No: TYL-2020-2009.en_US
dc.identifier.doi10.4274/MMJ.galenos.2024.27243
dc.identifier.endpage108en_US
dc.identifier.issn2149-2042
dc.identifier.issn2149-4606
dc.identifier.issue2en_US
dc.identifier.pmid38940481en_US
dc.identifier.scopus2-s2.0-85197874884en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage101en_US
dc.identifier.urihttps://doi.org/10.4274/MMJ.galenos.2024.27243
dc.identifier.urihttps://hdl.handle.net/20.500.14619/7609
dc.identifier.volume39en_US
dc.identifier.wosWOS:001274111400004en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofMedeniyet Medical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAstaxanthinen_US
dc.subjectrenalen_US
dc.subjectischemia/reperfusionen_US
dc.subjectoxidative stressen_US
dc.subjectautophagyen_US
dc.titleInvestigation of the Effect of Astaxanthin on Autophagy in Renal Ischemia-reper fusion Modeled Ratsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu