Improvement algorithm application with image processing interface software for the improvement of mini gel electrophoresis images
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Gazi Univ, Fac Engineering Architecture
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
It is aimed to carry out an experimental study by creating an algorithm function that enables the improvement of gel electrophoresis band images with the help of the Python programming language-based, mini gel electrophoresis system image processing interface. With this system, the analysis of gel images under UV (Ultraviolet) light was made with the designed interface software. The images were transferred to the interface software via the camera and filters were applied to highlight the lanes and bands. The two-term power function, which estimates BP (Base Pair) numbers with the most accurate result, was used. The software can be used on Raspberry Pi 3B+ by being integrated into the mini gel electrophoresis system via Python programming language with OpenCV library. In this study, gel images obtained from the mini gel electrophoresis system controlled by the embedded system were used to estimate the BP numbers of the bands in the images. By importing the gel images from the camera or from the file in the system, the interface software algorithm enabled the estimation of BP numbers in the manual measurements and reducing the average error rates to the range of 0.55% - 0.86%. In the interface software, which enables the calculation of lanes and BP numbers in gel images with the lowest error rate, two-term power function has been applied to the image processing algorithm instead of the two-term exponential function, based on the principle that exponential functions can also be defined as power functions. It has been revealed that the BP values calculated with this two-term power function work in accordance with its purpose with the value of R-2=0.9999533, which shows the closeness of the actual BP values.
Açıklama
Anahtar Kelimeler
Gel Electrophoresis, image processing, embedded systems, python user interface
Kaynak
Journal of the Faculty of Engineering and Architecture of Gazi University
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
37
Sayı
2
