The Role of Human Parvovirus B19 in the Pediatric Patients with Pancytopenia?

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dc.authoridAltay Kocak, Aylin/0000-0002-0451-0142
dc.authoridCOLAK, MERYEM/0000-0001-9876-935X
dc.authoridKocak, Ulker/0000-0002-8701-5285
dc.contributor.authorColak, Meryem
dc.contributor.authorKocak, Aylin A.
dc.contributor.authorDinc, Bedia
dc.contributor.authorKaya, Zuhre
dc.contributor.authorKocak, Ulker
dc.contributor.authorYenicesu, Idil
dc.contributor.authorBozdayi, Gulendam
dc.date.accessioned2024-09-29T16:10:15Z
dc.date.available2024-09-29T16:10:15Z
dc.date.issued2019
dc.departmentKarabük Üniversitesien_US
dc.description.abstractBackground: Parvoviruses are small DNA viruses causing erythema infectiosum, which is known as the fifth disease. The aim of this study was to investigate the presence of Parvovirus B19 DNA by Real-Time-PCR retrospectively in clinical samples of children diagnosed as acute leukemia and aplastic anemia when investigating the cause of pancytopenia and to investigate its relationship with the clinical manifestations. Methods: The study samples were collected between March 2014 and March 2018 in Gazi University, Faculty of Medicine, Department of Pediatric Hematology. Sixty pediatric patients; 37 males and 23 females, were included in the study. Nucleic acid isolation was performed by using MagNA-Pure Compact Nucleic Acid Isolation Kit (Roche, Germany). Extracted DNA was studied with LightCycler (R) 2.0 using the Real-Time PCR method and LightCycler (R) Parvovirus B19 Quantification Kit (Roche, Germany), and the results were evaluated quantitatively. Parvovirus B19 DNA detection interval of the kit was 10(1) - 10(6) copies/mL. Results: Sixty serum samples were investigated and 8.3% (5/60) Parvovirus B19 DNA positivity was determined. Of the five patients with Parvovirus B19 DNA positivity, three had acute lymphoblastic leukemia and two were diagnosed as aplastic anemia. Regarding viral load; 2/5, 1/5, 1/5, and 1/5 of the samples had a viral load of 10(2), 10(3), 104, and 105 copies/mL, respectively. Parvovirus B19 DNA positivity was detected in samples from March (2/5), April (2/5), and August (1/5). Conclusions: Patients with acute leukemia and aplastic anemia in childhood using immunosuppressive drugs, blood, and blood products during chemotherapy, encounter Parvovirus B19 infections in the follow-up period and are diagnosed by serological and molecular methods. As a result of the study, we suggest that the detection of Parvovirus B19 DNA by Real-Time PCR method in children being admitted with pancytopenia and diagnosed as acute leukemia and aplastic anemia is useful in the follow-up and treatment.en_US
dc.identifier.doi10.7754/Clin.Lab.2019.190311
dc.identifier.endpage2241en_US
dc.identifier.issn1433-6510
dc.identifier.issue12en_US
dc.identifier.pmid31850715en_US
dc.identifier.scopus2-s2.0-85076838600en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage2237en_US
dc.identifier.urihttps://doi.org/10.7754/Clin.Lab.2019.190311
dc.identifier.urihttps://hdl.handle.net/20.500.14619/8074
dc.identifier.volume65en_US
dc.identifier.wosWOS:000545454600004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherClin Lab Publen_US
dc.relation.ispartofClinical Laboratoryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectParvovirus B19en_US
dc.subjectreal-time polymerase chain reactio (Real-Time PCR)en_US
dc.subjectpediatric patienten_US
dc.subjectacute lymphoblastic leukemia (ALL)en_US
dc.subjectaplastic anemiaen_US
dc.titleThe Role of Human Parvovirus B19 in the Pediatric Patients with Pancytopenia?en_US
dc.typeArticleen_US

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