Biostimulation with diode laser positively regulates cementoblast functions, in vitro

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dc.authoridKAYIS, Seyit Ali/0000-0003-4791-8946
dc.authoridHAKKI, ERDOGAN ESREF/0000-0001-7147-7875
dc.authoridBozkurt, Serife Buket/0000-0002-8641-2844
dc.authoridHakki, Sema/0000-0001-8665-6235
dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorHakki, Erdogan E.
dc.contributor.authorKayis, Seyit Ali
dc.contributor.authorDundar, Niyazi
dc.contributor.authorHakki, Sema S.
dc.date.accessioned2024-09-29T15:51:08Z
dc.date.available2024-09-29T15:51:08Z
dc.date.issued2017
dc.departmentKarabük Üniversitesien_US
dc.description.abstractThe aim of this study was to evaluate the effects of diode laser biostimulation on cementoblasts (OCCM. 30). A total of 40 root plates were obtained from healthy third molar teeth and assigned to the following two groups: (1) control group and (2) laser-treated group. Root plates were placed into the cell culture inserts, and OCCM. 30 cells were seeded onto root plates. Cells were irradiated with a low level of diode laser (power: 0.3 W in continuous wave, 60 s/cm(2)). Proliferation and mineralized tissue-associated gene's and BMP's messenger RNA (mRNA) expressions of cementoblasts were evaluated. Total RNAs were isolated on day 3 and integrin-binding sialoprotein (Ibsp), bone gammacarboxyglutamate protein (Bglap), Type I collagen (Col1a1), osteoblastic transcription factor, runt-related transcription factor (Runx2), and Bone Morphogenetic Protein (BMP)-2, 3, 4, 6, and 7 mRNA expressions were determined using quantitative RT-PCR. von Kossa staining was performed to evaluate biomineralization of OCCM. 30 cells. In the proliferation experiment, while there was no significant difference until 96 h, laser irradiation retarded the decrease in cell proliferation trend after 96 h compared to the untreated control group. Statistically significant increase in Ibsp, Bglap, and BMP-2,3,6,7 mRNA expressions were noted in the laser groups when compared to the untreated control group (p < 0.05). Laser irradiation induced mineralized nodule formation of cementoblasts. The results of this study reveal that the biostimulation setting of diode laser modulates the behavior of cementoblasts inducing mineralized tissue-associated gene's mRNA expressions and mineralization. Therefore, biostimulation can be used during regenerative periodontal therapies to trigger cells with periodontal attachment apparatus.en_US
dc.description.sponsorshipSelcuk University Research Foundationen_US
dc.description.sponsorshipThis study was supported by Selcuk University Research Foundation. Grant was used for cell culture studies and SEM.en_US
dc.identifier.doi10.1007/s10103-017-2192-z
dc.identifier.endpage919en_US
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.issue4en_US
dc.identifier.pmid28332131en_US
dc.identifier.scopus2-s2.0-85015855175en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage911en_US
dc.identifier.urihttps://doi.org/10.1007/s10103-017-2192-z
dc.identifier.urihttps://hdl.handle.net/20.500.14619/3912
dc.identifier.volume32en_US
dc.identifier.wosWOS:000399885100022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer London Ltden_US
dc.relation.ispartofLasers in Medical Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCementumen_US
dc.subjectLaseren_US
dc.subjectBiostimulationen_US
dc.subjectRegenerationen_US
dc.subjectGene expressionen_US
dc.subjectMineralizationen_US
dc.titleBiostimulation with diode laser positively regulates cementoblast functions, in vitroen_US
dc.typeArticleen_US

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