A New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MS

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dc.contributor.authorKayili, Haci Mehmet
dc.contributor.authorAvci, Izzet
dc.contributor.authorSalih, Bekir
dc.date.accessioned2024-09-29T15:57:46Z
dc.date.available2024-09-29T15:57:46Z
dc.date.issued2019
dc.departmentKarabük Üniversitesien_US
dc.description.abstractEnrichment and/or purification of intact glycopeptides and derivatized glycans are essential for mass spectrometry-based glycoproteomics and glycomics because of analytical challenges observed in the analysis. Here, a titania-based material was synthesized using a facile sol-gel method for the efficient analysis of glycopeptides and glycans. The glycopeptide enrichment efficacy of the titania-based sol-gel material was evaluated by the enrichment of IgG N-glycopeptides from their proteolytic products. The selectivity and sensitivity (0.5 fmol mu L-1) of the titania-based sol-gel material was found to be quite high when compared with commercial TiO2. A pipette-tip application for rapid glycoproteomic and glycomic analysis was developed by packing titania-based material into the pipette tips. The released and derivatized N-glycans of human plasma, IgG and human transferrin were successfully purified by titania glyco-purification tips for the analysis by MALDI-MS. The enrichment performance of the titania glyco-purification tip was tested using human plasma digest as a real-world complex sample and, 112 N-glycopeptides from human plasma glycoproteins were detected by the analysis of enriched samples by nLC-QTOF-MS/MS. Finally, the structural characterization of the material was achieved in detail using various characterization approaches. (C) 2019 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTurkish Ministry of Development Project [FAY-2015-7201]; Turkish Academy of Science (TUBA)en_US
dc.description.sponsorshipThis study was partially supported by Turkish Ministry of Development Project with the project number: FAY-2015-7201. Bekir Salih gratefully acknowledges the Turkish Academy of Science (TUBA) for the partial financial support.en_US
dc.identifier.doi10.1016/j.jpba.2019.05.061
dc.identifier.endpage197en_US
dc.identifier.issn0731-7085
dc.identifier.issn1873-264X
dc.identifier.pmid31174130en_US
dc.identifier.scopus2-s2.0-85066444984en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage191en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2019.05.061
dc.identifier.urihttps://hdl.handle.net/20.500.14619/5008
dc.identifier.volume174en_US
dc.identifier.wosWOS:000479328000022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlycopeptideen_US
dc.subjectEnrichmenten_US
dc.subjectGlycanen_US
dc.subjectPurificationen_US
dc.subjectGlycoproteomicsen_US
dc.subjectGlycomicsen_US
dc.subjectMassen_US
dc.subjectSpectrometryen_US
dc.titleA New titania glyco-purification tip for the fast enrichment and efficient analysis of glycopeptides and glycans by MALDI-TOF-MSen_US
dc.typeArticleen_US

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