Biomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineering

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dc.authoridTezcaner, Aysen/0000-0003-4292-5856
dc.authoridMOTAMENI, ALI/0000-0001-7400-9890
dc.authoridISIK ERTOP, Gulhan/0000-0002-2327-8916
dc.authoridEvis, Zafer/0000-0002-7518-8162
dc.authoridBILGIN, Saliha/0000-0002-3382-3156
dc.authoridAlshemary, Ammar Z/0000-0001-5367-1869
dc.contributor.authorAlshemary, Ammar Z.
dc.contributor.authorBilgin, Saliha
dc.contributor.authorIsik, Gulhan
dc.contributor.authorMotameni, Ali
dc.contributor.authorTezcaner, Aysen
dc.contributor.authorEvis, Zafer
dc.date.accessioned2024-09-29T15:57:42Z
dc.date.available2024-09-29T15:57:42Z
dc.date.issued2021
dc.departmentKarabük Üniversitesien_US
dc.description.abstractBiocompatible dicalcium phosphate (DCP) cements are widely used as bone repair materials. In this study, we aimed to investigate the impact of different amounts of sodium alginate (SA) on the microstructural, mechanical, and biological properties of DCP cements. Beta-tricalcium phosphate (?-TCP) was prepared using a microwaveassisted wet precipitation system. Lattice parameters of the obtained particles determined from X-ray diffraction (XRD), were in good match with a standard phase of ?-TCP. Scanning electron microscopy (SEM) examination revealed that the particles were in globular shape. Furthermore, all functional groups of ?-TCP were also detected using Fourier-transform infrared spectroscopy (FTIR) spectra. DCP cement (pure phase) was synthesized using monocalcium phosphate monohydrate (MCPM)/?-TCP powder mixture blended with 1.0 mL of water. SA/DCP cement composites were synthesized by dissolving different amounts of SA into water (1.0 mL) to obtain different final concentrations (0.5%, 1%, 2% and 3%). The prepared cements were characterized with XRD, SEM, FTIR and Thermogravimetric analysis (TGA). XRD results showed that pure DCP and SA/DCP cements were in a good match with Monetite phase. SEM results confirmed that addition of SA inhibited the growth of DCP particles. Setting time and injectability behaviour were significantly improved upon increasing the SA amount into DCP cements. In vitro biodegradation was evaluated using Simulated body fluid (SBF) over 21 days at 37 ?C. The highest cumulative weight loss (%) in SBF was observed for 2.0% SA/DCP (about 26.52%) after 21 days of incubation. Amount of Ca2+ ions released in SBF increased with the addition of SA. DCP and SA/DCP cements showed the highest mechanical strength after 3 days of incubation in SBF and declined with prolonged immersion periods. In vitro cell culture experiments were conducted using Dental pulp stem cells (DPSCs). Viability and morphology of cells incubated in extract media of DCP and SA/DCP discs after 24 h incubation was studied with MTT assay and fluorescence microscopy imaging, respectively. All cements were cytocompatible and viability of cells incubated in extracts of cements was higher than observed in the control group. Based on the outcomes, SA/DCP bone cements have a promising future to be utilized as bone filler.en_US
dc.description.sponsorshipKarabuk University [KB?BAP18YL178]en_US
dc.description.sponsorshipDr. Ammar Z. Alshemary would like to thank Karabuk University for providing financial support via Project no. KB?BAP18YL178.en_US
dc.identifier.doi10.1016/j.jmbbm.2021.104439
dc.identifier.issn1751-6161
dc.identifier.issn1878-0180
dc.identifier.pmid33691231en_US
dc.identifier.scopus2-s2.0-85101975851en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.jmbbm.2021.104439
dc.identifier.urihttps://hdl.handle.net/20.500.14619/4960
dc.identifier.volume118en_US
dc.identifier.wosWOS:000647763500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of the Mechanical Behavior of Biomedical Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDicalcium phosphate cementen_US
dc.subjectSodium alginateen_US
dc.subjectSetting timeen_US
dc.subjectBiomechanicsen_US
dc.subjectIn vitro cell Culture analysisen_US
dc.titleBiomechanical Evaluation of an Injectable Alginate / Dicalcium Phosphate Cement Composites for Bone Tissue Engineeringen_US
dc.typeArticleen_US

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