Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release

Basit Öğe Kaydı
dc.authoridKaryelioglu, Melda/0000-0003-1918-0964
dc.authoridKARAV, SERCAN/0000-0003-4056-1673
dc.authoridKaplan, Merve/0000-0002-3322-0988
dc.authoriderturk, melih/0000-0003-4140-6325
dc.contributor.authorSucu, Berfin
dc.contributor.authorBayraktar, Ayse
dc.contributor.authorDuman, Hatice
dc.contributor.authorArslan, Aysenur
dc.contributor.authorKaplan, Merve
dc.contributor.authorKaryelioglu, Melda
dc.contributor.authorNtelitze, Eda
dc.date.accessioned2024-09-29T16:08:17Z
dc.date.available2024-09-29T16:08:17Z
dc.date.issued2021
dc.departmentKarabük Üniversitesien_US
dc.description.abstractProtein glycosylation is a diverse and common post-translational modification that has been associated with many important roles such as protein function, including protein folding, stability, enzymatic protection, and biological recognition. N-glycans attached to glycoproteins (such as lactoferrin, lactadherin, and immunoglobulins) cannot be digested by the host and reach the large intestine, where they are consumed by certain beneficial microbes. Therefore, they are considered nextgeneration prebiotic compounds that can selectively stimulate the gut microbiome's beneficial microorganisms. However, the isolation of these new classes of prebiotics requires novel enzymes. Here, we describe the recombinant production of novel glycosidases from different Bifidobacteria strains (isolated from infants, rabbits, chicken, and bumblebee) for improved N-glycan isolation from glycoproteins. The method presented in this study includes the following steps: molecular cloning of Bifidobacterial genes by an in vivo recombinational cloning strategy, control of transformation success, protein induction, and protein purification.en_US
dc.description.sponsorshipTUBITAK [118z146]en_US
dc.description.sponsorshipThis study is supported by TUBITAK #118z146 and Uluova Sut Ticaret A.S (Uluova Milk Trading Co.).en_US
dc.identifier.doi10.3791/62804
dc.identifier.issn1940-087X
dc.identifier.issue173en_US
dc.identifier.pmid34369921en_US
dc.identifier.scopus2-s2.0-85113890033en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.3791/62804
dc.identifier.urihttps://hdl.handle.net/20.500.14619/7470
dc.identifier.wosWOS:000682796200091en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJournal Of Visualized Experimentsen_US
dc.relation.ispartofJove-Journal of Visualized Experimentsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLinked Glycansen_US
dc.subjectGlycoproteinsen_US
dc.subjectOligosaccharidesen_US
dc.subjectDeglycosylationen_US
dc.subjectAcetylglucosaminidaseen_US
dc.subjectGlycosylationen_US
dc.subjectRevealsen_US
dc.titleRecombinant Production of Bifidobacterial Endoglycosidases for N-glycan Releaseen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu