Gene expression profiles of some cytokines, growth factors, receptors, and enzymes (GM-CSF, IFN?, MMP-2, IGF-II, EGF, TGF-?, IGF-IIR) during pregnancy in the cat uterus

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dc.authoridKAYIS, Seyit Ali/0000-0003-4791-8946
dc.authoridAgaoglu, Ali Reha/0000-0002-6545-8800
dc.contributor.authorAgaoglu, Ozgecan Korkmaz
dc.contributor.authorAgaoglu, Ali Reha
dc.contributor.authorGuzeloglu, Aydin
dc.contributor.authorAsian, Selim
dc.contributor.authorKurar, Ercan
dc.contributor.authorKayis, Seyit Ali
dc.contributor.authorSchaefer-Somi, Sabine
dc.date.accessioned2024-09-29T16:00:49Z
dc.date.available2024-09-29T16:00:49Z
dc.date.issued2016
dc.departmentKarabük Üniversitesien_US
dc.description.abstractEarly pregnancy is one of the most critical periods of pregnancy, and many factors such as cytokines, enzymes, and members of the immune system have to cooperate in a balanced way. In the present study, the gene expression profiles of factors associated with pregnancy such as EGF, transforming growth factor beta, granulocyte-macrophage colony stimulating factor, interferon gamma, insulin-like growth factor 2, insulin-like growth factor 2 receptor, and matrix metalloproteinase 2 were analyzed in uterine tissues of female cats. The cats were assigned to five groups: G1 (embryo positive, n = 7; 7th day after mating), G2 (after implantation, n = 7; 20th day after mating), G3 (midgestation, n = 7; 24-25th day after mating), G4 (late gestation, n = 7; 30-45th day after mating), G5 (oocyte group, n = 7; 7th day after estrus). Tissue samples from the uterus and placenta were collected after ovariohysterectomy. Relative messenger RNA levels were determined by real-time polymerise chain reaction. All the factors examined were detected in all tissue samples. In the course of pregnancy, significantly higher expression of EGF and matrix metalloproteinase 2 in G2 than in G1 was observed (P < 0.05). Insulin-like growth factor 2 expression was higher in all groups than in G1 (P < 0.05). Upregulation of EGF during implantation was detected. The expression of interferon gamma was significantly higher in G3 than in G1 (P < 0.05). Transforming growth factor beta and granulocyte macrophage colony-stimulating factor were constantly expressed in all groups. In conclusion; the expressions of these factors in feline uterine tissue at different stages of pregnancy might indicate that these factors play roles in the development of pregnancy such as trophoblast invasion, vascularization, implantation, and placentation. (C) 2016 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112O878]en_US
dc.description.sponsorshipThis study was financed by the Scientific and Technological Research Council of Turkey (TUBITAK; project No.112O878, the project coordinator is Dr Oka). This study was presented in the 18th European Society of Domestic Animal Reproduction, Helsinki, Finland.en_US
dc.identifier.doi10.1016/j.theriogenology.2015.10.001
dc.identifier.endpage644en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue4en_US
dc.identifier.pmid26559469en_US
dc.identifier.scopus2-s2.0-84960349314en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage638en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2015.10.001
dc.identifier.urihttps://hdl.handle.net/20.500.14619/5375
dc.identifier.volume85en_US
dc.identifier.wosWOS:000370111500009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFelineen_US
dc.subjectPregnancyen_US
dc.subjectCytokineen_US
dc.subjectGrowth factoren_US
dc.subjectReceptoren_US
dc.titleGene expression profiles of some cytokines, growth factors, receptors, and enzymes (GM-CSF, IFN?, MMP-2, IGF-II, EGF, TGF-?, IGF-IIR) during pregnancy in the cat uterusen_US
dc.typeArticleen_US

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