The protective role of N-acetylcysteine against acrylamide-induced genotoxicity and oxidative stress in rats
Küçük Resim Yok
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Gene Therapy and Molecular Biology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Acrylamide is neurotoxic, genotoxic and highly carcinogenic in humans and animals. The aim of this study was to research the protective role of N-acetylcysteine against acrylamide-induced genotoxicity and oxidative stress in rats. In the present study, male wistar albino rats were divided into four groups, each containing 10 as follows: Control (C) group, Acrylamide (AA) group, N-acetylcysteine (NAC) group, Acrylamide + N-acetylcysteine (AA+NAC) group. At the end of 21 days, Malondialdehyde (MDA) and Glutathione (GSH) levels were measured in plasma and DNA damage was observed in lymphocytes. Plasma GSH level decreased significantly in AA group compared to C group (P < 0.05). However, GSH level increased significantly in AA+NAC group compared to AA group (p< 0.05). MDA levels increased significantly in AA group compared to C group (p< 0.05). But AA+NAC administration decreased MDA levels as compared to AA group (p< 0.05). Comet analysis results showed that lymphocyte DNAs were normal appearance in C and NAC groups. DNA damage was observed at a highest level in AA group. Furthermore some lymphocytes exhibited apoptotic appearance. However this damage was prevented by NAC administration on lymphocyte DNAs significantly. Our results demonstrated that high level of AA caused oxidative stress and DNA damage, but NAC could have a protective role on acrylamide-induced toxicity.
Açıklama
Anahtar Kelimeler
Acrylamide, Genotoxicity, Lymphocyte, N-acetylcysteine, Oxidative stress
Kaynak
Gene Therapy and Molecular Biology
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
16
Sayı
1
