Disposable biosensor based on ionic liquid, carbon nanofiber and poly (glutamic acid) for tyramine determination

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dc.authoridOKMAN KOCOGLU, Irem/0000-0001-6071-9795
dc.contributor.authorKocoglu, Irem Okman
dc.contributor.authorErden, Pinar Esra
dc.contributor.authorKilic, Esma
dc.date.accessioned2024-09-29T15:54:54Z
dc.date.available2024-09-29T15:54:54Z
dc.date.issued2024
dc.departmentKarabük Üniversitesien_US
dc.description.abstractIn this study, an electrochemical biosensor based on carbon nanofibers (CNF), ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (IL), poly(glutamic acid) (PGA) and tyrosinase (Tyr) modified screen printed carbon electrode (SPE) was constructed for tyramine determination. Optimum experimental parameters such as CNF and IL amount, polymerization conditions of glutamic acid, enzyme loading, pH of test solution and operating potential were explored. The construction steps of the Tyr/PGA/CNF-IL/SPE were pursued by scanning electron microscopy and cyclic voltammetry. The Tyr/PGA/CNF-IL/SPE biosensor exhibited linear response to tyramine in the range of 2.0 x 10-7 - 4.8 x 10-5 M with a low detection limit of 9.1 x 10-8 M and sensitivity of 302.6 mu A mM-1. The other advantages of Tyr/PGA/CNF-IL/SPE include its high reproducibility, good stability and anti-interference ability. The presented biosensor was also applied for tyramine determination in malt drink and pickle juice samples and mean analytical recoveries of spiked tyramine were calculated as 100.6% and 100.4% respectively.en_US
dc.description.sponsorshipKarabuk University Scientific Research Projects Coordination Unit [KBBAP-22-ABP-077]en_US
dc.description.sponsorshipThis study is supported by Karabuk University Scientific Research Projects Coordination Unit. Project Number: KBUEBAP-22-ABP-077.en_US
dc.identifier.doi10.1016/j.ab.2023.115387
dc.identifier.issn0003-2697
dc.identifier.issn1096-0309
dc.identifier.pmid37951456en_US
dc.identifier.scopus2-s2.0-85178539588en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1016/j.ab.2023.115387
dc.identifier.urihttps://hdl.handle.net/20.500.14619/4343
dc.identifier.volume684en_US
dc.identifier.wosWOS:001115620300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmperometryen_US
dc.subjectBiosensoren_US
dc.subjectTyramineen_US
dc.subjectCarbon nanofiberen_US
dc.subjectIonic liquiden_US
dc.subjectPolyglutamic aciden_US
dc.titleDisposable biosensor based on ionic liquid, carbon nanofiber and poly (glutamic acid) for tyramine determinationen_US
dc.typeArticleen_US

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