Molecular Epidemiological Analysis of Bacillus Pseudo-Outbreak due to Contaminated Culture Tubes Containing Stuart Medium

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dc.authoridOtlu, Baris/0000-0002-6220-0521
dc.contributor.authorAsgin, Nergis
dc.contributor.authorKal-Cakmakliogullari, Elcin
dc.contributor.authorOtlu, Baris
dc.contributor.authorErsoy, Omer F.
dc.contributor.authorCelik, Betul
dc.contributor.authorBasustaoglu, Ahmet
dc.date.accessioned2024-09-29T16:10:15Z
dc.date.available2024-09-29T16:10:15Z
dc.date.issued2022
dc.departmentKarabük Üniversitesien_US
dc.descriptionInternational DNA Day and Genome Congress (IDDGC) -- APR 24-28, 2017 -- Ahi Evran Univ, Kirsehir, TURKEYen_US
dc.description.abstractBackground: It is challenging to determine whether Bacillus species other than Bacillus anthracis cause infections. Pseudo and true outbreaks of Bacillus spp. have been noted. Here, we present a molecular analysis of a Bacillus spp. pseudo-outbreak caused by contaminated culture tubes containing Stuart medium. Methods: Between January and March 2015, a high percentage of Bacillus spp. was isolated from the wound samples of inpatients at the Karabuk University Hospital, and an outbreak was suspected. Environmental and staff nasal samples were cultured aerobically, and Bacillus spp. were isolated from some of them. However, the isolation of Bacillus spp. in throat cultures of outpatients suggested contamination caused by culture tubes containing Stuart medium. We examined two lots of culture tubes used in the hospital. Although the culture tubes' expiry date and storage conditions were suitable, Bacillus spp. grew in one of these lots. A total of 47 Bacillus spp. isolated during this period were identified, and the clonal relationship among the isolates was investigated by arbitrarily primed polymerase chain reaction. Results: Twenty-seven strains were identified as Bacillus megaterium and 20 as Bacillus firmus. Of the four strains isolated from the Stuart medium, two were identified as B. firmus and the other two were B. megaterium. Two B. firmus strains isolated from the Stuart medium and two B. firmus strains obtained from the coronary intensive care environmental samples were matched and clustered within the same genotype. We recalled all culture tubes containing Stuart medium. After another brand's culture tubes were distributed, no growth was observed. It was then understood that the pseudo-outbreak source was contaminated culture tubes containing Stuart medium. Conclusions: Microbiological controls of medical materials and equipment should be regularly checked to prevent outbreaks (true or pseudo).en_US
dc.identifier.doi10.7754/Clin.Lab.2021.210306
dc.identifier.endpage120en_US
dc.identifier.issn1433-6510
dc.identifier.issue1en_US
dc.identifier.pmid35023676en_US
dc.identifier.scopus2-s2.0-85123302987en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage115en_US
dc.identifier.urihttps://doi.org/10.7754/Clin.Lab.2021.210306
dc.identifier.urihttps://hdl.handle.net/20.500.14619/8075
dc.identifier.volume68en_US
dc.identifier.wosWOS:000744051400014en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherClin Lab Publen_US
dc.relation.ispartofClinical Laboratoryen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectarbitrarily primed polymerase chain reactionen_US
dc.subjectBacillus firmusen_US
dc.subjectBacillus megateriumen_US
dc.subjectmolecular typingen_US
dc.subjectpseudo-outbreaken_US
dc.titleMolecular Epidemiological Analysis of Bacillus Pseudo-Outbreak due to Contaminated Culture Tubes Containing Stuart Mediumen_US
dc.typeConference Objecten_US

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